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PLA2G10 antibody (A07633) performs well in Flow Cytometry, IP, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of CACO-2 cells using anti-PLA2G10 antibody (A07633).
Overlay histogram showing CACO-2 cells stained with A07633 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLA2G10 Antibody (A07633, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with A07633 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLA2G10 Antibody (A07633, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PLA2G10 Antibody Picoband®
PLA2G10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, WB |
Tested Applications
PLA2G10 antibody was positively detected by WB in:
human MCF-7 whole cell, human Caco-2 whole cell, human A431 whole cell, human PC-3 whole cell, rat testis tissue, mouse testis tissue, mouse small intestine tissue, Caco-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PLA2G10 antibody was positively detected by IHC in:
human colon cancer tissue, human stomach cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PLA2G10 antibody was positively detected by IF in:
human colon cancer tissue, human stomach cancer tissue
PLA2G10 antibody was positively detected by FC in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PLA2G10 Antibody Picoband®
View all PLA2G10 Antibodies
View all PLA2G10 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PLA2G10.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
18-20 kDa
Calculated Molecular Weight
18.2 kDa
Background of PLA2G10
This gene encodes a member of the phospholipase A2 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This calcium-dependent enzyme hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In one example, this enzyme catalyzes the release of arachidonic acid from cell membrane phospholipids, thus playing a role in the production of various inflammatory lipid mediators, such as prostaglandins. The encoded protein may promote the survival of breast cancer cells through its role in lipid metabolism.
Cite This Product
Anti-PLA2G10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07633)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Rabbit
85%
Moderate Homology
Cow
80%
Moderate Homology
Sheep
80%
Moderate Homology
Dog
80%
Moderate Homology
Mouse
75%
Moderate Homology
Rat
70%
Moderate Homology
Zebrafish
70%
Moderate Homology
Drosophila
45%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PLA2G10 antibody
Immunohistochemistry (IHC) protocol for PLA2G10 antibody
Immunofluorescence (IF) protocol for PLA2G10 antibody
Flow Cytometry (FC) protocol for PLA2G10 antibody
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Product Promise
We promise Anti-PLA2G10 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A07633
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 27, 2026