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- Table of Contents
PROM1 antibody (A01767-3) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Flow Cytometry analysis of CACO-2 cells using anti-PROM1 antibody (A01767-3).
Overlay histogram showing CACO-2 cells stained with A01767-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROM1 Antibody (A01767-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with A01767-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROM1 Antibody (A01767-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PROM1 Antibody Picoband®
PROM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
PROM1 antibody was positively detected by WB in:
human placenta tissue, human CACO-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PROM1 antibody was positively detected by ICC/IF in:
A549 cell
PROM1 antibody was positively detected by FC in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunocytochemistry/Immunofluorescence
4μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-PROM1 Antibody Picoband®
View all PROM1 Antibodies
View all PROM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PROM1 recombinant protein (Position: D27-R821).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
97 kDa
Calculated Molecular Weight
97.2 kDa
Background of PROM1
Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.
Cite This Product
Anti-PROM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01767-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Rabbit
67%
Low homology
Dog
66%
Low homology
Rat
60%
Low homology
Mouse
60%
Low homology
Cow
55%
Low homology
Sheep
55%
Low homology
Zebrafish
44%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PROM1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PROM1 antibody
Flow Cytometry (FC) protocol for PROM1 antibody
Publications
Application:
Species:
TMEM165 promotes glioblastoma progression through epithelial-mesenchymal transition-mediated invasion and temozolomide chemoresistance
Published: 2025-11-25
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.50
Species: Human
PMID: 41308767
The CD133+CXCR4+ Colorectal Tumor Cells Promote Colorectal Cancer Progression by PI3K/AKT Signaling
Published: 2022-05-16
Journal: JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
Application: IHC
Impact Factor: 3.66
Species: Human
PMID: 35377243
Identification of miRNA signature associated with BMP2 and chemosensitivity of TMZ in glioblastoma stem-like cells
Published: 2019-09-09
Journal: Genes & Diseases
Application: WB
Impact Factor: 0.00
Species: Human
PMID: 32884997
CD146 is highly expressed in glioma stem cells and acts as a cell cycle regulator
Published: 2019-05-30
Journal: JOURNAL OF NEURO-ONCOLOGY
Application: IHC
Impact Factor: 3.13
Species: Human
PMID: 31147892
Identification and differentiation therapy strategy of pterygium in vitro
Published: 2018-08-15
Journal: American Journal of Translational Research
Application: IF
Impact Factor: 3.06
Species: Mouse
PMID: 30210698
The impact of bone marrow-derived mesenchymal stem cells on neovascularisation in rats with brain injury
Published: 2018-06-28
Journal: FOLIA NEUROPATHOLOGICA
Application: FC
Impact Factor: 1.35
Species: Rat
PMID: 30509031
Effects of all-trans retinoic acid on goat dermal papilla cells cultured in vitro
Published: 2018-05-15
Journal: ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Application: ICC
Impact Factor: 1.88
Species: Goat
Effects of 5‑hydroxy‑4'‑nitro‑7‑propionyloxy‑genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells
Published: 2018-02-02
Journal: Oncology Letters
Application: IF
Impact Factor: 1.66
Species: Goat
PMID: 29552162
Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections
Published: 2017-10-31
Journal: Stem Cells International
Application: IF
Impact Factor: 3.54
Species: Human
PMID: 29225627
β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells
Published: 2014-05-19
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: IF,WB
Impact Factor: 2.77
Species: Human
PMID: 24841897
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Product Promise
We promise Anti-PROM1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01767-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026