Anti-RNAse H2A Antibody

Product Name

Anti-RNAse H2A Antibody

View all RNASEH2A Antibodies

SKU/Catalog Number

A07386

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RNAse H2A Antibody (Catalog # A07386). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RNAse H2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RNAse H2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07386)

Host

Rabbit

Contents

RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A. The immunogen is located within amino acids 190 - 240 of RNAse H2A.

Reactive Species

A07386 is reactive to RNASEH2A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.4 kDa

Background of RNASEH2A

Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07386 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RNASEH2A

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