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Product Info Summary
| SKU: | A02049 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RuvB-like 1 RUVBL1 Antibody
SKU/Catalog Number
A02049
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-RuvB-like 1 RUVBL1 Antibody (Catalog# A02049). Tested in IHC, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-RuvB-like 1 RUVBL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02049)
Host
Rabbit
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant RuvB-like 1 protein (amino acids 1-268)
Cross-reactivity
Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, horse, rhesus macaque, dog, chimpanzee.
Reactive Species
A02049 is reactive to RUVBL1 in Human
Calculated molecular weight
50.2 kDa
Background of RUVBL1
NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02049 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 0.5-1 µg/ml
IHC: 10 µg/ml
Validation Images & Assay Conditions
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Immunohistochemical analysis of RuvB-like 1. Anti-RuvB-like 1 antibody in human Testis tissue at 10 &mu
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blot analysis of RuvB-like 1. Anti-RuvB-like 1 antibody was used at 1 &mu
Specific Publications For Anti-RuvB-like 1 RUVBL1 Antibody (A02049)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-RuvB-like 1 RUVBL1 Antibody
Question
I see that the anti-RuvB-like 1 antibody A02049 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-15
Question
Is there a BSA free version of anti-RuvB-like 1 antibody A02049 available?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RuvB-like 1 antibody A02049 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-15
Question
Is this A02049 anti-RuvB-like 1 antibody reactive to the isotypes of RUVBL1?
K. Patel
Verified customer
Asked: 2018-01-04
Answer
The immunogen of A02049 anti-RuvB-like 1 antibody is A partial length recombinant RuvB-like 1 protein (amino acids 1-268). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-04
Question
We are currently using anti-RuvB-like 1 antibody A02049 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
The anti-RuvB-like 1 antibody (A02049) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-15
Question
I was wanting to use your anti-RuvB-like 1 antibody for IHC for human bone marrow on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bone marrow identification?
T. Jackson
Verified customer
Asked: 2015-09-08
Answer
As indicated on the product datasheet, A02049 anti-RuvB-like 1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-08
Question
See below the WB image, lot number and protocol we used for bone marrow using anti-RuvB-like 1 antibody A02049. Please let me know if you require anything else.
M. Edwards
Verified customer
Asked: 2014-03-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-18
