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- Table of Contents
RUVBL1 antibody (A02049-2) performs well in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Hela cells using anti-TIP49A/RUVBL1 antibody (A02049-2).
Overlay histogram showing Hela cells stained with A02049-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with A02049-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TIP49A/RUVBL1 Antibody Picoband®
RUVBL1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
RUVBL1 antibody was positively detected by WB in:
human K562 whole cell, human 293T whole cell, human HT1080 whole cell, monkey COS-7 whole cell, human MCF-7 whole cell, human Daudi whole cell, human MOLT-4 whole cell, human HEL whole cell, rat testis tissue, rat C6 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RUVBL1 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human thyroid cancer tissue, human lung adenocarcinoma tissue, human spleen tissue, human tonsil tissue, human ovarian cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
RUVBL1 antibody was positively detected by ICC/IF in:
A549 cell
RUVBL1 antibody was positively detected by IF in:
human colon cancer tissue
RUVBL1 antibody was positively detected by IP in:
K562 cell
RUVBL1 antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-TIP49A/RUVBL1 Antibody Picoband®
View all RUVBL1 Antibodies
View all RUVBL1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TIP49A/RUVBL1 recombinant protein (Position: Q13-D23).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
50.2 kDa
Background of RUVBL1
RuvB-like 1 (E. coli), also known as RUVBL1 and TIP49, is a human gene. This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.
Cite This Product
Anti-TIP49A/RUVBL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02049-2)
Protocols
Western Blot protocol for RUVBL1 antibody
Immunohistochemistry (IHC) protocol for RUVBL1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RUVBL1 antibody
Immunofluorescence (IF) protocol for RUVBL1 antibody
Immunoprecipitation (IP) protocol for RUVBL1 antibody
Flow Cytometry (FC) protocol for RUVBL1 antibody
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Product Promise
We promise Anti-TIP49A/RUVBL1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02049-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026