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SERPINB3 antibody (M04067-2) performs well in IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of SerpinB3 using anti-SerpinB3 antibody (M04067-2).
SerpinB3 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:400 rabbit anti-SerpinB3 Antibody (M04067-2) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SerpinB3 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:400 rabbit anti-SerpinB3 Antibody (M04067-2) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-SerpinB3 Rabbit Monoclonal Antibody
SERPINB3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, IHC, ICC, WB |
Tested Applications
SERPINB3 antibody was positively detected by WB in:
A431 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-SerpinB3 Rabbit Monoclonal Antibody
View all SERPINB3 Antibodies
View all SERPINB3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
26S64
Isotype
IgG
Immunogen
A synthesized peptide derived from SerpinB3
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
39 kDa
Calculated Molecular Weight
44.6 kDa
Cite This Product
Anti-SerpinB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04067-2)
Protocols
Western Blot protocol for SERPINB3 antibody
Review
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1 Review For Anti-SerpinB3 Rabbit Monoclonal Antibody
In this IHC experiment using Anti-SERPINB3 antibody (Cat# M04067-2) on human cervical cancer paraffin sections, SERPINB3 was highly expressed in tumor cells with clear positive staining and low background, demonstrating excellent antibody performance.
| SKU | M04067-2 |
|---|---|
| Application | Immunohistochemistry |
| Sample | human cervical cancer tissue |
| Sample Processing Description | Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded |
| Other Reagents | Goat serum, DAB substrate solution |
| Primary Antibody | SerpinB3 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:400, overnight at 4 ℃ |
| Secondary Antibody | Two-step IHC detection kit |
| Secondary Incubation | 30 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | The results demonstrated high expression of SERPINB3 in cervical cancer tumor cells with clear positive staining; further studies will compare its expression levels between tumor and adjacent non-tumor tissues. |
Juan Wang, The First Affiliated Hospital of Shihez
Verified customer
Submitted 2026-02-25
Product Promise
We promise Anti-SerpinB3 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M04067-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026