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Product Info Summary
| SKU: | A03782 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Shank 2 Antibody
SKU/Catalog Number
A03782
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Shank 2 Antibody catalog # A03782. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Shank 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03782)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SHANK2. AA range:331-380
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03782 is reactive to SHANK2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
158.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03782 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western Blot analysis of RAW264.7 cells using Shank 2 Polyclonal Antibody
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Western blot analysis of lysates from RAW264.7, Jurkat, HepG2, and COLO cells, using SHANK2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using SHANK2 antibody.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Shank 2 Antibody (A03782)
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6 Customer Q&As for Anti-Shank 2 Antibody
Question
We are currently using anti-Shank 2 antibody A03782 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The anti-Shank 2 antibody (A03782) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-20
Question
I see that the anti-Shank 2 antibody A03782 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-08
Question
I would like to test anti-Shank 2 antibody A03782 on human dorsolateral prefrontal cortex for research purposes, then I may be interested in using anti-Shank 2 antibody A03782 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The products we sell, including anti-Shank 2 antibody A03782, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-14
Question
Does anti-Shank 2 antibody A03782 work on horse WB with dorsolateral prefrontal cortex?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Our lab technicians have not tested anti-Shank 2 antibody A03782 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Shank 2 antibody A03782 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse dorsolateral prefrontal cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
Is this A03782 anti-Shank 2 antibody reactive to the isotypes of SHANK2?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
The immunogen of A03782 anti-Shank 2 antibody is Synthesized peptide derived from the Internal region of human Shank 2. at AA rangle: 300-380. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-25
Question
My question regarding product A03782, anti-Shank 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Brown
Verified customer
Asked: 2015-06-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03782 anti-Shank 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-05
