Anti-Shank 2 Antibody

SHANK2 antibody

Boster Bio Anti-Shank 2 Antibody catalog # A03782. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A03782
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Shank 2 Antibody

View all SHANK2 Antibodies

SKU/Catalog Number

A03782

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Shank 2 Antibody catalog # A03782. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Shank 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03782)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2F1

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Shank 2. at AA rangle: 300-380

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03782 is reactive to SHANK2 in Human

Applications

A03782 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

158.822kDa

Background of SHANK2

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For SHANK2 (Source: Uniprot.org, NCBI)

Gene Name

SHANK2

Full Name

SH3 and multiple ankyrin repeat domains protein 2

Weight

158.822kDa

Superfamily

SHANK family

Alternative Names

AUTS17; cortactin binding protein 1; cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CORTBP1; CORTBP1CTTNBP1; CTTNBP1; GKAP/SAPAP interacting protein; KIAA1022; proline-rich synapse associated protein 1; Proline-rich synapse-associated protein 1; ProSAP1; SH3 and multiple ankyrin repeat domains 2; SH3 and multiple ankyrin repeat domains protein 2; SHANK; SHANK2; SPANK-3 SHANK2 AUTS17, CORTBP1, CTTNBP1, ProSAP1, SHANK, SPANK-3 SH3 and multiple ankyrin repeat domains 2 SH3 and multiple ankyrin repeat domains protein 2|GKAP/SAPAP interacting protein|cortactin SH3 domain-binding protein|cortactin-binding protein 1|proline-rich synapse associated protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SHANK2, check out the SHANK2 Infographic

SHANK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SHANK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Shank 2 Antibody

Question

We are currently using anti-Shank 2 antibody A03782 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

The anti-Shank 2 antibody (A03782) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-20

Question

I see that the anti-Shank 2 antibody A03782 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-08

Question

I would like to test anti-Shank 2 antibody A03782 on human dorsolateral prefrontal cortex for research purposes, then I may be interested in using anti-Shank 2 antibody A03782 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

The products we sell, including anti-Shank 2 antibody A03782, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-14

Question

Does anti-Shank 2 antibody A03782 work on horse WB with dorsolateral prefrontal cortex?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

Our lab technicians have not tested anti-Shank 2 antibody A03782 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Shank 2 antibody A03782 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse dorsolateral prefrontal cortex in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-21

Question

Is this A03782 anti-Shank 2 antibody reactive to the isotypes of SHANK2?

Verified Customer

Verified customer

Asked: 2018-01-25

Answer

The immunogen of A03782 anti-Shank 2 antibody is Synthesized peptide derived from the Internal region of human Shank 2. at AA rangle: 300-380. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-25

Question

My question regarding product A03782, anti-Shank 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Brown

Verified customer

Asked: 2015-06-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03782 anti-Shank 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-06-05

Order DetailsPrice
A03782

100uL

$399

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