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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Liquid (sterile filtered)
Boster Bio Anti-SMAD2 Antibody catalog # A00090. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Cite This Product
Anti-SMAD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00090)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A00090 is reactive to SMAD2 in Human, Mouse, Rat
A00090 is guaranteed for IP, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blot: 1:1,000 - 1:3,000
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SMAD2.
Gene/Protein Information For SMAD2 (Source: Uniprot.Org, NCBI)
Mothers against decapentaplegic homolog 2
hMAD-2; hSMAD2; JV18; JV18-1SMAD, mothers against DPP homolog 2 (Drosophila); MAD homolog 2; Mad protein homolog; Mad, mothers against decapentaplegic homolog 2; MADH2MGC34440; MADR2MGC22139; Mad-related protein 2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila); SMAD, mothers against DPP homolog 2; Smad2; Smad2/3 SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SMAD2, check out the SMAD2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SMAD2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00090 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mouse hepatic neoplasm formation induced by trace level and low frequency exposure to diethylnitrosamine through %u03B2-catenin signaling pathway
Astragaloside effect on TGF-%u03B21, SMAD2/3, and %u03B1-SMA expression in the kidney tissues of diabetic KKAy mice
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
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