Anti-Toll-like receptor 5 TLR5 Antibody

Product Name

Anti-Toll-like receptor 5 TLR5 Antibody

View all TLR5 Antibodies

SKU/Catalog Number

A00462-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Toll-like receptor 5 TLR5 Antibody (Catalog # A00462-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Toll-like receptor 5 TLR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00462-1)

Host

Rabbit

Contents

TLR5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5. The immunogen is located within the last 50 amino acids of TLR5.

Reactive Species

A00462-1 is reactive to TLR5 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

97.8 kDa

Background of TLR5

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-κB, leading to the activation of TNF-α and other cytokines. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires'disease and demonstrates the importance of TLR5 in the innate immune response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00462-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TLR5 antibody can be used for detection of TLR5 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TLR5

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