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- Table of Contents
TUBB3 antibody (M01857-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Immunofluorescent analysis of A431 cells, using beta III Tubulin Antibody .
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Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
TUBB3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
TUBB3 antibody was positively detected by WB in:
human SH-SY5Y whole cell, human SIHA whole cell, rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TUBB3 antibody was positively detected by IHC in:
human testicular seminoma tissue, human testicular seminoma tissue, human thyroid papillary carcinoma tissue, human thyroid papillary carcinoma tissue, human tonsil tissue, human tonsil tissue, human urothelial carcinoma tissue, human urothelial carcinoma tissue, non-small cell lung cancer tissue, non-small cell lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TUBB3 antibody was positively detected by ICC/IF in:
A431 cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
View all TUBB3 Antibodies
View all TUBB3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta III Tubulin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
50.4 kDa
Cite This Product
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01857-1)
Protocols
Western Blot protocol for TUBB3 antibody
Immunohistochemistry (IHC) protocol for TUBB3 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TUBB3 antibody
Publications
Application:
Species:
Adipose-derived stem cell-conditioned medium mitigates ischemia-induced neuronal injury via the JAK1/STAT3 signaling pathway
Published: 2026-02-23
Journal: Frontiers in Cellular Neuroscience
Application: IF
Impact Factor: 4.00
Species: Rat
PMID: 41809974
Carboxymethyl chitosan hydrogel synergistically regulates neural stem cells with ultrasound for the treatment of traumatic brain injury
Published: 2025-10-13
Journal: Materials Today Advances
Application: IF
Impact Factor: 8.00
Species: Mouse
Nociceptor neurons promote PDAC progression and cancer pain by interaction with cancer-associated fibroblasts and suppression of natural killer cells
Published: 2025-03-24
Journal: CELL RESEARCH
Application: IF
Impact Factor: 25.90
Species: Human,Mouse
PMID: 40122998
Epidural administration of large dose of opioid μ receptor agonist may impair cardiac functions and myocardial viability via desensitizing transient receptor potential vanilloid 1
Published: 2024-01-04
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: IF
Impact Factor: 3.30
Species: Rat
PMID: 38184280
Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury
Published: 2023-10-21
Journal: MOLECULAR NEUROBIOLOGY
Application: IF
Impact Factor: 5.10
Species: Rat
PMID: 37864767
Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity
Published: 2023-07-20
Journal: Journal of Neural Engineering
Application: IF
Impact Factor: 4.00
Species: Human
PMID: 37433290
Mechanism behind the neuronal ephaptic coupling during synchronizing by specific brain-triggered wave as neuronal motor toolkit
Published: 2021-02-11
Journal: Scientific Reports
Application: WB
Impact Factor: 4.38
Species: Rat
PMID: 33574428
3D bioprinted glioma stem cells for brain tumor model and applications of drug susceptibility
Published: 2016-10-10
Journal: Biofabrication
Application: IHC
Impact Factor: 4.70
Species: Human
PMID: 27725343
Review
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Product Promise
We promise Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01857-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026