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Product Info Summary
| SKU: | A07183-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-UBAP2L Antibody
SKU/Catalog Number
A07183-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-UBAP2L Antibody (Catalog # A07183-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
UBAP2L antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-UBAP2L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07183-1)
Host
Rabbit
Contents
UBAP2L Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
UBAP2L antibody was raised against a 19 amino acid peptide from near the amino terminus of human UBAP2L. The immunogen is located within amino acids 200 - 250 of UBAP2L.
Cross-reactivity
UBAP2L antibody is human and mouse reactive. At least four isoforms of UBAP2L are known to exist; this antibody will detect all four isoforms. This antibody is predicted to not cross-react with UBAP2.
Reactive Species
A07183-1 is reactive to UBAP2L in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
114.5 kDa
Background of UBAP2L
The UBA domain-containing protein ubiquitin associated protein 2-like (UBAP2L), also known as NICE4, is a ubiquitously expressed 1,087 amino acid protein. UBAP2L is preferentially expressed in hematopoietic stem cell (HSC) populations and interacts with BMI1, a well-known determinant of HSC function (1). It is essential for the activity of long-term repopulating HSCs but not the BMI1-dependent repression of the Ink4a/Arf locus (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07183-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.
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Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.
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Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.
Red: UBAP2L Antibody (A07183-1)
Blue: DAPI staining
Specific Publications For Anti-UBAP2L Antibody (A07183-1)
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