Product Info Summary
SKU: | M05329 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-VAPA Mouse Monoclonal Antibody [Clone ID: OTI10E10]
SKU/Catalog Number
M05329
Size
100 µl
Description
Boster Bio VAPA mouse monoclonal antibody, clone OTI10E10 (formerly 10E10). Catalog# M05329. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-VAPA Mouse Monoclonal Antibody [Clone ID: OTI10E10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05329)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI10E10
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 2-227 of human VAPA (NP_919415) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05329 is reactive to VAPA in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
27.893kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05329 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAPA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VAPA (1:2000).
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Western blot analysis of extracts (10ug) from 9 different cell lines by using anti-VAPA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VAPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Protein Target Info & Infographic
Gene/Protein Information For VAPA (Source: Uniprot.org, NCBI)
Gene Name
VAPA
Full Name
Vesicle-associated membrane protein-associated protein A
Weight
27.893kDa
Superfamily
VAMP-associated protein (VAP) (TC 9.B.17) family
Alternative Names
hVAP-33; MGC3745,33 kDa VAMP-associated protein; VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa; VAMP-A; VAP-33; VAP33VAMP (vesicle-associated membrane protein)-associated protein A (33kD); VAPA; VAP-A; VAP-AVAMP-associated protein A; vesicle-associated membrane protein-associated protein A VAPA VAMP-A, VAP-33, VAP-A, VAP33, hVAP-33 VAMP associated protein A vesicle-associated membrane protein-associated protein A|33 kDa VAMP-associated protein|VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VAPA, check out the VAPA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VAPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VAPA Mouse Monoclonal Antibody [Clone ID: OTI10E10] (M05329)
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