Anti-VIP peptides VIP Antibody

Boster Bio Anti-VIP peptides VIP Antibody catalog # RP1108. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: RP1108
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC

Product Name

Anti-VIP peptides VIP Antibody

See all VIP products

SKU/Catalog Number

RP1108

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VIP peptides VIP Antibody catalog # RP1108. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VIP peptides VIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1108)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human VIP (81-107aa HADGVFTSDFSKLLGQLSAKKYLESLM), different from the related mouse and rat sequences by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1108 is reactive to VIP in Human, Mouse, Rat

Applications

RP1108 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For VIP (Source: Uniprot.Org, NCBI)

Uniprot ID

P01282

Gene ID

7432

Gene Name

VIP

Full Name

VIP peptides

Weight

19.169kDa

Superfamily

glucagon family

Alternative Names

MGC13587; PHM27; vasoactive intestinal peptide; VIP peptides; VIP VIP PHM27 vasoactive intestinal peptide VIP peptides|prepro-VIP

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VIP, check out the VIP Infographic

VIP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VIP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1108 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Kawashima M,Yajima T,Tachiya D,Kokubun S,Ichikawa H,Sato T. Parasympathetic neurons in the human submandibular ganglion. Tissue Cell. 2021 Jan 20;70:101496.doi:10.1016/j.tice.2021. 101496.Epub ahead of print.PMID:33517097.
Species: Human
RP1108 usage in article: APP:IHC, SAMPLE:FEMORAL ARTERY, DILUTION:1:5000

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

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16 Customer Q&As for Anti-VIP peptides VIP Antibody

Question

Our lab were content with the WB result of your anti-VIP antibody. However we have seen positive staining in pancreatic carcinoma secreted. using this antibody. Is that expected? Could you tell me where is VIP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

Based on literature, pancreatic carcinoma does express VIP. Generally VIP expresses in secreted. Regarding which tissues have VIP expression, here are a few articles citing expression in various tissues:
Pancreatic carcinoma, Pubmed ID: 6139527
Pheochromocytoma, Pubmed ID: 1318039
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-05-01

Question

My question regarding product RP1108, anti-VIP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1108 anti-VIP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-28

Question

I see that the anti-VIP antibody RP1108 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-13

Question

I was wanting to use your anti-VIP antibody for IHC for rat fundus of stomach on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fundus of stomach identification?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

It shows on the product datasheet, RP1108 anti-VIP antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fundus of stomach in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-13

Question

What are the suggestion concentrations for IHC and IF applications?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

The suggested concentration for immunohistochemistry is 1ug/mL and for immuno fluorescence is 1-2ug/mL

Boster Scientific Support

Answered: 2019-12-06

Question

I would like to test anti-VIP antibody RP1108 on rat fundus of stomach for research purposes, then I may be interested in using anti-VIP antibody RP1108 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Moore

Verified customer

Asked: 2019-12-05

Answer

The products we sell, including anti-VIP antibody RP1108, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-05

Question

We have seen staining in human fundus of stomach. Are there any suggestions? Is anti-VIP antibody supposed to stain fundus of stomach positively?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

From literature fundus of stomach does express VIP. From Uniprot.org, VIP is expressed in fundus of stomach, prostate, pancreatic carcinoma, pheochromocytoma, among other tissues. Regarding which tissues have VIP expression, here are a few articles citing expression in various tissues:
Pancreatic carcinoma, Pubmed ID: 6139527
Pheochromocytoma, Pubmed ID: 1318039
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-02

Question

Does anti-VIP antibody RP1108 work for IHC with fundus of stomach?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

According to the expression profile of fundus of stomach, VIP is highly expressed in fundus of stomach. So, it is likely that anti-VIP antibody RP1108 will work for IHC with fundus of stomach.

Boster Scientific Support

Answered: 2019-10-18

Question

Is a blocking peptide available for product anti-VIP antibody (RP1108)?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

We do provide the blocking peptide for product anti-VIP antibody (RP1108). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-24

Question

Do you have a BSA free version of anti-VIP antibody RP1108 available?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-VIP antibody RP1108 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-14

Question

We are currently using anti-VIP antibody RP1108 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

The anti-VIP antibody (RP1108) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-24

Question

Anti-VIP Antibody RP1108 I am interested in VIP antibody and I wonder compatibility this Antibody for ELISA assay? Has the antibody been tested for ELISA application?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

RP1108 has not been tested for ELISA application. According to the immunogen sequence, it will not likely to work for ELISA.

Boster Scientific Support

Answered: 2018-12-14

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fundus of stomach using anti-VIP antibody RP1108. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-10-01

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-01

Question

Is this RP1108 anti-VIP antibody reactive to the isotypes of VIP?

Verified Customer

Verified customer

Asked: 2018-09-04

Answer

The immunogen of RP1108 anti-VIP antibody is A synthetic peptide corresponding to a sequence in the middle region of human VIP (81-107aa HADGVFTSDFSKLLGQLSAKKYLESLM), different from the related mouse and rat sequences by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-04

Question

Would RP1108 anti-VIP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

You can see on the product datasheet, RP1108 anti-VIP antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-07-11

Question

See below the WB image, lot number and protocol we used for fundus of stomach using anti-VIP antibody RP1108. Please let me know if you require anything else.

G. Li

Verified customer

Asked: 2018-01-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-02

Conjugation

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Ships directly to USA and Canada. For other regions, visit our distributors page

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RP1108
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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