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- Table of Contents
VIP antibody (RP1108) performs well in IF, IHC and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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IF analysis of VIP using anti-VIP antibody (RP1108).
VIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
VIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-VIP peptides VIP Antibody
VIP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC |
Tested Applications
VIP antibody was positively detected by IHC in:
human pancreatic cancer tissue, rat pancreas tissue, rat intestine tissue, mouse pancreas tissue, mouse intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
VIP antibody was positively detected by IF in:
rat colon tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunofluorescence
5 μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Rat
Tested
Tested
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-VIP peptides VIP Antibody
View all VIP Antibodies
View all VIP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human VIP, different from the related mouse and rat sequences by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
19.2 kDa
Background of VIP
Vasoactive intestinal peptide, also known as PHM27 or VIP, is a peptide hormone containing 28 amino acid residues. This gene is mapped to 6q25. The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
Cite This Product
Anti-VIP peptides VIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1108)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
93%
High Homology
Dog
93%
High Homology
Cow
89%
Moderate Homology
Sheep
89%
Moderate Homology
Mouse
85%
Moderate Homology
Rat
85%
Moderate Homology
Zebrafish
78%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for VIP antibody
Immunofluorescence (IF) protocol for VIP antibody
Publications
Application:
Species:
The Sinuvertebral Nerve Revisited: A Morphological and Immunohistochemical Study
Published: 2026-07-09
Journal: Life-Basel
Application: IHC
Impact Factor: 3.90
Species: Human
PMID: 42514207
Cellular- and systems-level profiling of amyloid-beta effects on circadian timing
Published: 2026-04-12
Journal: NEUROBIOLOGY OF DISEASE
Application: IF
Impact Factor: 5.60
Species: Mouse
PMID: 41980665
Dynamic Regulation of Vasoactive Intestinal Polypeptide in the Retina of Guinea Pigs with Experimentally Induced Myopia
Published: 2025-11-03
Journal: EXPERIMENTAL EYE RESEARCH
Application: IHC
Impact Factor: 2.70
Species: Guinea pig
PMID: 41192575
The human major sublingual gland and its neuropeptidergic and nitrergic innervations
Published: 2024-05-29
Journal: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
Application: IHC
Impact Factor: 2.00
Species: Human
PMID: 38821428
The role of vasoactive intestinal peptide (VIP) in atropine-related inhibition of the progression of myopia
Published: 2024-01-26
Journal: BMC Ophthalmology
Application: IHC
Impact Factor: 1.70
Species: Guinea pig
PMID: 38279089
Innervation of the human minor salivary glands; immunohistochemical study for neurochemical substances
Published: 2022-09-14
Journal: TISSUE & CELL
Application: IHC
Impact Factor: 2.59
Species: Human
PMID: 36126414
Distribution of neuronal cells which contain dopamine β-hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human internal carotid nerve
Published: 2021-09-05
Journal: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
Application: IHC
Impact Factor: 2.06
Species: Rabbit
PMID: 34486238
Parasympathetic neurons in the human submandibular ganglion
Published: 2021-01-20
Journal: TISSUE & CELL
Application: IHC
Impact Factor: 2.47
Species: Human
PMID: 33517097
Sensory neurons in the human jugular ganglion
Published: 2020-02-09
Journal: TISSUE & CELL
Application: IHC
Impact Factor: 1.84
Species: Human
PMID: 32473709
Distribution of postganglionic neurons which contain dopamine β-hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human middle cervical ganglion
Published: 2019-04-16
Journal: TISSUE & CELL
Application: IHC
Impact Factor: 1.55
Species: Human
PMID: 31133245
S100+ cells: A new neuro-immune cross-talkers in lymph organs
Published: 2013-01-23
Journal: Scientific Reports
Application: IF
Impact Factor: 2.93
Species: Rat
PMID: 23346371
The structural characteristics and expression of neuropeptides in the esophagus of patients with congenital esophageal atresia and tracheoesophageal fistula
Published: 2007-08-14
Journal: JOURNAL OF PEDIATRIC SURGERY
Application: IHC
Impact Factor: 1.11
Species: Human
PMID: 17706510
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Product Promise
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RP1108
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026