Anti-HSD17B2 Antibody

Rabbit Polyclonal HSD17B2 Antibody. Validated in IHC, WB and tested in Human.

SKU PA1604
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-HSD17B2 Antibody
See all HSD17B2 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1604
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal HSD17B2 Antibody. Validated in IHC, WB and tested in Human. Size: 100μg/vial.
Cite This Product Anti-HSD17B2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1604)
Specificity Anti-HSD17B2 Antibody (PA1604) reacts with Human HSD17B2, in native form and recombinant. Superfamily members of HSD17B2 are not reactive to PA1604.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-HSD17B2 Antibody (PA1604) was raised against A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2(373-387aa MPRALRMPNYKKKAT)..
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-HSD17B2 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-HSD17B2 Antibody (PA1604).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/pa1604 1 WB anti hsd17b2 antibody.jpg

Anti-HSD17B2 antibody, PA1604, Western blotting
All lanes: Anti HSD17B2 (PA1604) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 43KD
Observed bind size: 43KD

/antibody/pa1604 2 IHC anti hsd17b2 antibody.jpg

Anti-HSD17B2 antibody, PA1604, IHC(P)
IHC(P): Human Placenta Tissue

/antibody/pa1604 3 IHC anti hsd17b2 antibody.jpg

Anti-HSD17B2 antibody, PA1604, IHC(F)
IHC(F): Human Placenta Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P37059
Gene Name HSD17B2
Protein Name Estradiol 17-beta-dehydrogenase 2
Alternative Names Estradiol 17-beta-dehydrogenase 2;1.1.1.62;17-beta-hydroxysteroid dehydrogenase type 2;17-beta-HSD 2;20 alpha-hydroxysteroid dehydrogenase;20-alpha-HSD;E2DH;Microsomal 17-beta-hydroxysteroid dehydrogenase;Short chain dehydrogenase/reductase family 9C member 2;Testosterone 17-beta-dehydrogenase;1.1.1.239;HSD17B2;EDH17B2, SDR9C2;Show more
Subcellular Localization Membrane ; Single-pass type II membrane protein .
Molecular Weight 42785 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
Research Areas Cancer, Growth Factors, Growth Factors/Hormones, Signal Transduction

*You can search these to find other products in these research areas.
Background HSD17B2(17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42(43%) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-HSD17B2 Antibody (PA1604)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1604)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.