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Product Info Summary
| SKU: | AZQ6NZ09 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Zebrafish ADRM1 Antibody Picoband®
SKU/Catalog Number
AZQ6NZ09
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish ADRM1 Antibody Picoband® catalog # AZQ6NZ09. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish ADRM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6NZ09)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish ADRM1 recombinant protein (Position: S14-M385).
Reactive Species
AZQ6NZ09 is reactive to ADRM1 in Zebrafish
Observed Molecular Weight
42 kDa
Background of ADRM1
Proteasomal ubiquitin receptor ADRM1 is a protein that in humans is encoded by the ADRM1 gene. This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ6NZ09 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Positive Control
WB: zebrafish head tissue, female zebrafish viscera tissue, male zebrafish viscera tissue, whole zebrafish tissue, zebrafish embryo tissue
IHC: zebrafish brain tissue
Validation Images & Assay Conditions
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Western blot analysis of ADRM1 using anti-ADRM1 antibody (AZQ6NZ09).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: female zebrafish viscera tissue lysates,
Lane 3: male zebrafish viscera tissue lysates,
Lane 4: whole zebrafish tissue lysates,
Lane 5: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRM1 antigen affinity purified polyclonal antibody (AZQ6NZ09) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADRM1 at approximately 42 kDa. The expected band size for ADRM1 is at 42 kDa.
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IHC analysis of ADRM1 using anti-ADRM1 antibody (AZQ6NZ09).
ADRM1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADRM1 Antibody (AZQ6NZ09) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish ADRM1 Antibody Picoband® (AZQ6NZ09)
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