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FSHB antibody (AZQ6TCF7) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of FSHB using anti-FSHB antibody (AZQ6TCF7).
FSHB was detected in a paraffin-embedded section of zebrafish hypophysis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSHB Antibody (AZQ6TCF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
FSHB was detected in a paraffin-embedded section of zebrafish hypophysis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSHB Antibody (AZQ6TCF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish FSHB Antibody Picoband®
FSHB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Tested Applications
FSHB antibody was positively detected by WB in:
zebrafish head tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FSHB antibody was positively detected by IHC in:
zebrafish hypophysis tissue, zebrafish ovary tissue, zebrafish testis tissue, zebrafish eye tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish FSHB Antibody Picoband®
View all FSHB Antibodies
View all FSHB Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FSHB recombinant protein (Position: E19-H130)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of FSHB
Enables hormone activity. Acts upstream of or within ovarian follicle development; sex determination; and spermatogenesis. Predicted to be located in extracellular region. Predicted to be active in cytoplasm and extracellular space. Is expressed in several structures, including brain; endocrine system; female organism; kidney; and male organism. Human ortholog(s) of this gene implicated in amenorrhea; hypogonadotropic hypogonadism 23 with or without anosmia; and hypogonadotropic hypogonadism 24 without anosmia. Orthologous to several human genes including CGB1 (chorionic gonadotropin subunit beta 1); CGB2 (chorionic gonadotropin subunit beta 2); and CGB3 (chorionic gonadotropin subunit beta 3).
Cite This Product
Anti-Zebrafish FSHB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6TCF7)
Protocols
Western Blot protocol for FSHB antibody
Immunohistochemistry (IHC) protocol for FSHB antibody
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Product Promise
We promise Anti-Zebrafish FSHB Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
AZQ6TCF7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026