Click image to see more details
Product Info Summary
| SKU: | AZA4IGC8 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Zebrafish P5CS/ALDH18A1 Antibody
SKU/Catalog Number
AZA4IGC8
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish P5CS/ALDH18A1 Antibody catalog # AZA4IGC8. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish P5CS/ALDH18A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA4IGC8)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish P5CS/ALDH18A1 recombinant protein (Position: R35-A204).
Reactive Species
AZA4IGC8 is reactive to ALDH18A1 in Zebrafish
Background of ALDH18A1
Delta-1-pyrroline-5-carboxylate synthetase (P5CS) is an enzyme that in humans is encoded by the ALDH18A1 gene. This gene is mapped to 10q24.1. This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate,a critical step in the de novo biosynthesis of proline,ornithine and arginine. Mutations in this gene lead to hyperammonemia,hypoornithinemia,hypocitrullinemia,hypoargininemia and hypoprolinemia and may be associated with neurodegeneration,cataracts and connective tissue diseases. Alternatively spliced transcript variants,encoding different isoforms,have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA4IGC8 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Positive Control
IHC: zebrafish kidney tissue
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of P5CS/ALDH18A1 using anti-P5CS/ALDH18A1 antibody (AZA4IGC8).
P5CS/ALDH18A1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P5CS/ALDH18A1 Antibody (AZA4IGC8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish P5CS/ALDH18A1 Antibody (AZA4IGC8)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Zebrafish P5CS/ALDH18A1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Zebrafish P5CS/ALDH18A1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
