ABAT Antibodies

4-aminobutyrate aminotransferase, mitochondrial (ABAT)

Catalyzes the conversion of gamma-aminobutyrate and L- beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

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Gene Information

Human
Gene Name:	ABAT
Uniprot:	P80404
Entrez:	18

Family

Belongs to:
class-III pyridoxal-phosphate-dependent aminotransferase family

Alternative Names

(S)-3-amino-2-methylpropionate transaminase; 4-aminobutyrate aminotransferase; 4-aminobutyrate aminotransferase, mitochondrial; 4-aminobutyrate transaminase; EC 2.6.1.19; EC 2.6.1.22; FLJ17813; FLJ30272; G; GABA aminotransferase; GABA transaminase; GABA transferase; GABA-AT; GABA-T; GABATNPD009; Gamma-amino-N-butyrate transaminase; hCG1984265; L-AIBAT

Molecular Weight

Mass (kDA):
56.439 kDA

Genomic Context

Human
Location:	16p13.2
Sequence:	16; NC_000016.10 (8674617..8784575)

Tissue Specificity

Liver > pancreas > brain > kidney > heart > placenta.

Subcellular Localizations

Mitochondrion matrix.

Diseases

• Dysequilibrium Syndrome
• Schizophrenia
• Epilepsy
• Cholestasis
• Autistic Disorder
• Stenosis
• Ingestion Poison
• Sarcoma
• Dyskinetic Syndrome
• Delayed Hypersensitivity
• Dyskinesia, Drug-induced

• Paranoid Schizophrenia
• Rheumatoid Arthritis
• Epilepsy, Temporal Lobe
• Involuntary Movements
• Rhabdomyosarcoma, Embryonal
• Alveolar Rhabdomyosarcoma

Pathways

• Secretion
• Transport
• Dna Replication
• Synaptic Transmission
• Mapk Cascade
• Hypersensitivity

• Pathogenesis

PTMs

• Phosphorylation

• Deacetylation

• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ABAT

Best Uses Of The ABAT Marker

Antibodies to ABAT can be used to study ABAT signaling in the mouse or rat. These antibodies are highly specific and have been extensively used in many scientific applications. Boster antibodies have been cited in numerous publications over the past 25 years, and they have been rigorously tested on Western Blotting, Immunohistochemistry, and ELISA. Boster has the answer for you, whether you want to perform an immunoblotting experiment using a particular antibody or to determine the presence ABAT protein.

Anti-ABAT Signer

You may be interested in analyzing Anti-ABAT's role in your research. Boster bio has the Anti-ABAT marker. The antibody binds Cattle ABAT and reacts to the receptor. The Boster bio Anti-ABAT Marker is used in various applications including ELISA, Western Blot, and Enzyme-Linked Immunosorbent Assay.

Boster Bio was established in 1993. It specializes in manufacturing antibodies. The company is well known for its signature products. The company recently expanded its product line to include PCR-related molecular biological products. Boster Bio offers many services for scientists, including free technical support and 24 hour access. This helps scientists with their research. Customer support can be reached via email, chat, and phone.

Primary antibodies of high-affinity

Using high-affinity primary antibodies can speed up your research. Boster Bio makes a variety antibodies that are valid for IHC/WB, ELISA/FC, and immunoprecipitation. These antibodies are also available to be used in human and mouse samples. BosterBio antibodies are designed to maintain biological activities and high-quality standards. All BosterBio products come with a free secondary antigen.

You must ensure that your primary antibodies work as efficiently as possible by being highly specific. Boster's anti-beta actin rabbit monoclonal antibody was used as an internal control in western blotting experiments. Boster used antibeta Actin as a primary antibodies against the retinol-binding protein in this study.

Boster also offers monoclonal and polyclonal primary antibody, in addition to being extremely specific. The latter are highly versatile and are cited in scientific journals and publications around the world. Boster's high-affinity prima antibodies have been consistently ranked as one of the best-selling research reagents for over 25 year. The company's highly specific antibodies are particularly useful when studying gene expression or protein levels of tissues and cells.

Boster Bio's high affinity primary antibodies have another advantage: they can separate the primary from secondary antibodies. This allows researchers more questions to be asked about specimens. It also provides contextual data and robust answers. This can help you get better results and increase productivity. These antibodies can be used for a variety of purposes, including testing antigens in a wide range.

To make high-affinity primaries antibodies, single B cells must first be sorted into 96 well PCR plates with an RNase inhibit. Next, V H and Vk genes were amplified using nested PCR reactions. Specific PCR primers were created to incorporate restriction sites in the VH and Vk gene genes. Once this was completed, the membrane was washed with a phenol-chloroform buffer.

These antibodies can be recombinant, making them immune-compatible with various cell lines. Recombinant antibodies are less sensitive to cell-line variation and lot-to–lot variability. They can therefore guarantee maximum antigen specificity. They can also save money. These antibodies are highly sensitive and specific, which means they're an excellent choice for research.

Immunoblotting with mice and rats

Antibodies for western-blotting are specifically designated for this application. They should also list "western Blotting" on their labels. Primary antibodies should be specifically designated for specific applications and should be used in separate experiments in order to detect each protein target. The primary antibody should be derived from a different species, preferably one that is distantly related. Primaries antibodies should not be derived from mouse and rat, goat and sheep, for they may cross-react, and cause confusion.

A rabbit polyclonal immunoglobulin was used to detect ABAT proteins in the brains of rat and mouse. It also detects GABAA receptor B2/3 and p Subunits in rat kidney tissues. Cross-reactivity is also demonstrated with human samples. Customers who give feedback are offered incentives by the company. These results suggest that ABAT Protein is present in the brains and kidneys, and is involved during GABA metabolism.

Western blotting, which is based on ELISA, allows for multiple proteins to be detected instead of one. A gel electophoresis allows for semi-quantification and size of target proteins. This allows you to compare protein concentrations between samples by using an external quantity standard. It is an effective tool that allows for the detection of protein in different samples.

Western blot uses antibodies to identify the proteins in samples. Antibodies recognize proteins through binding to epitopes, which are highly specific protein sequences. Western blotting can be used to identify a protein and to quantify its levels. The process begins with a sample being applied to a gel electrophoresis matrix and then a primary antibody is used to the samples.

Applications

There are 125 different questions in the ABAT Examination. 100 of these are live questions and 25 are pretest questions. These questions cannot be marked. They can however be used to study gene expression in cells. Because it regulates BLBC aggression, ABAT is an important marker for cancer research. There are several applications of the ABAT marker. We will discuss some of these in this article.

ABAT expression in breast cancer patients is a useful independent prognostic indicator. ABAT expression may determine which breast cancer patients would benefit from chemotherapy and avoid the side effects. It may also be useful in predicting the outcome of a patient's chemotherapy treatment. However, more research is needed to determine ABAT expression's prognostic importance. Below, we will discuss some of the uses of ABAT in cancer research.

The ABAT marker acts as a tumor suppressor gene. It has been shown in vivo that it can suppress tumorigenicity. After injecting 1x106 MDAMB-231 cells or a control cell, the researchers conducted a soft-agar test on female SCID mice. Tumor formation was observed every two to 4 days. Eight mice were used to measure tumor size and weight.

Snail inhibits ABAT expression in several breast cancer cell line lines. This is likely because Snail suppresses ABAT through transcriptional repression. These studies show that ABAT is an important cancer marker. These findings suggest that ABAT could be a useful biomarker to detect breast cancer. Although this research is still in the early stages of development, it is already showing promise as a breast cancer researcher.