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Product Info Summary
| SKU: | PB10019 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-ABAT Antibody Picoband®
SKU/Catalog Number
PB10019
PB1071 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABAT Antibody Picoband® catalog # PB10019. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. ABAT (4-aminobutyrate aminotransferase) is described as the enzyme responsible for GABA catabolism to succinic semialdehyde; the active enzyme is a pyridoxal-5′-phosphate–complexed homodimer. Assay context: antibody validated for Flow Cytometry, IF, ICC, and WB; reactive to ABAT in human/mouse/rat; immunogen is E. coli–derived human ABAT recombinant protein (K388–K500) with reported observed MW ~54 kDa (calculated 56.4 kDa) and stated no cross-reactivity. In cell-state studies, ABAT abundance can be interpreted alongside stress/adaptation nodes such as CRYAB (proteostasis; putative) and ATG5 (autophagy; putative); flow applications often require fluorophore tagging (see educational context on antibody conjugation), while cellular protein quantitation can be complemented by in-cell western blot readouts (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10019)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10019 is reactive to ABAT in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
56.4 kDa
Background of ABAT
4-Aminobutyrate aminotransferase is a protein that in humans is encoded by the ABAT gene. ABAT is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5- phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of humans ynapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractoryseizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10019 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
ICC/IF: MCF-7 cell
FCM: CACO-2 cell
Validation Images & Assay Conditions
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Western blot analysis of ABAT using anti-ABAT antibody (PB10019).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABAT antigen affinity purified polyclonal antibody (Catalog # PB10019) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABAT at approximately 54 kDa. The expected band size for ABAT is at 54 kDa.
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IF analysis of ABAT using anti-ABAT antibody (PB10019).
ABAT was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABAT Antibody (PB10019) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of CACO-2 cells using anti-ABAT antibody (PB10019).
Overlay histogram showing CACO-2 cells stained with PB10019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABAT Antibody (PB10019, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-ABAT Antibody Picoband® (PB10019)
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6 Customer Q&As for Anti-ABAT Antibody Picoband®
Question
Is this PB10019 anti-ABAT antibody reactive to the isotypes of ABAT?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The immunogen of PB10019 anti-ABAT antibody is E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-09
Question
I have a question about product PB10019, anti-ABAT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10019 anti-ABAT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ABAT antibody PB10019. Let me know if you need anything else.
C. Anderson
Verified customer
Asked: 2016-12-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-22
Question
Would anti-ABAT antibody PB10019 work on pig WB with pancreatic islet?
A. Taylor
Verified customer
Asked: 2016-05-12
Answer
Our lab technicians have not tested anti-ABAT antibody PB10019 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ABAT antibody PB10019 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig pancreatic islet in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-12
Question
We are currently using anti-ABAT antibody PB10019 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
S. Carter
Verified customer
Asked: 2015-09-18
Answer
The anti-ABAT antibody (PB10019) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-18
Question
Would anti-ABAT antibody PB10019 work for WB with liver?
S. Patel
Verified customer
Asked: 2013-01-15
Answer
According to the expression profile of liver, ABAT is highly expressed in liver. So, it is likely that anti-ABAT antibody PB10019 will work for WB with liver.
Boster Scientific Support
Answered: 2013-01-15
