Product Info Summary
SKU: | PB10019 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ABAT Antibody Picoband™
SKU/Catalog Number
PB10019
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABAT Antibody Picoband™ catalog # PB10019. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABAT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10019)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10019 is reactive to ABAT in Human, Mouse, Rat
Applications
PB10019 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
56.439kDa
Background of ABAT
4-Aminobutyrate aminotransferase is a protein that in humans is encoded by the ABAT gene. ABAT is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5- phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of humans ynapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractoryseizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABAT using anti-ABAT antibody (PB10019).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABAT antigen affinity purified polyclonal antibody (Catalog # PB10019) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABAT at approximately 54 kDa. The expected band size for ABAT is at 54 kDa.
Click image to see more details
Figure 2. IF analysis of ABAT using anti-ABAT antibody (PB10019).
ABAT was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABAT Antibody (PB10019) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-ABAT antibody (PB10019).
Overlay histogram showing CACO-2 cells stained with PB10019 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABAT Antibody (PB10019, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ABAT (Source: Uniprot.org, NCBI)
Gene Name
ABAT
Full Name
4-aminobutyrate aminotransferase, mitochondrial
Weight
56.439kDa
Superfamily
class-III pyridoxal-phosphate-dependent aminotransferase family
Alternative Names
(S)-3-amino-2-methylpropionate transaminase; 4-aminobutyrate aminotransferase; 4-aminobutyrate aminotransferase, mitochondrial; 4-aminobutyrate transaminase; EC 2.6.1.19; EC 2.6.1.22; FLJ17813; FLJ30272; G; GABA aminotransferase; GABA transaminase; GABA transferase; GABA-AT; GABA-T; GABATNPD009; Gamma-amino-N-butyrate transaminase; hCG1984265; L-AIBAT ABAT GABA-AT, GABAT, NPD009 4-aminobutyrate aminotransferase 4-aminobutyrate aminotransferase, mitochondrial|(S)-3-amino-2-methylpropionate transaminase|4-aminobutyrate transaminase|GABA aminotransferase|GABA transaminase|GABA transferase|gamma-amino-N-butyrate transaminase|gamma-aminobutyrate aminotransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABAT, check out the ABAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABAT Antibody Picoband™ (PB10019)
Hello CJ!
No publications found for PB10019
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ABAT Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-ABAT Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-ABAT Antibody Picoband™
Question
Is this PB10019 anti-ABAT antibody reactive to the isotypes of ABAT?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The immunogen of PB10019 anti-ABAT antibody is E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-09
Question
I have a question about product PB10019, anti-ABAT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10019 anti-ABAT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ABAT antibody PB10019. Let me know if you need anything else.
C. Anderson
Verified customer
Asked: 2016-12-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-22
Question
Would anti-ABAT antibody PB10019 work on pig WB with pancreatic islet?
A. Taylor
Verified customer
Asked: 2016-05-12
Answer
Our lab technicians have not tested anti-ABAT antibody PB10019 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ABAT antibody PB10019 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig pancreatic islet in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-12
Question
We are currently using anti-ABAT antibody PB10019 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
S. Carter
Verified customer
Asked: 2015-09-18
Answer
The anti-ABAT antibody (PB10019) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-18
Question
Would anti-ABAT antibody PB10019 work for WB with liver?
S. Patel
Verified customer
Asked: 2013-01-15
Answer
According to the expression profile of liver, ABAT is highly expressed in liver. So, it is likely that anti-ABAT antibody PB10019 will work for WB with liver.
Boster Scientific Support
Answered: 2013-01-15