Anti-ABAT Antibody Picoband™

ABAT antibody

Boster Bio Anti-ABAT Antibody Picoband™ catalog # PB10019. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10019
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-ABAT Antibody Picoband™

View all ABAT Antibodies

SKU/Catalog Number

PB10019

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ABAT Antibody Picoband™ catalog # PB10019. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABAT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10019)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10019 is reactive to ABAT in Human, Mouse, Rat

Applications

PB10019 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

56.439kDa

Background of ABAT

4-Aminobutyrate aminotransferase is a protein that in humans is encoded by the ABAT gene. ABAT is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5- phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of humans ynapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractoryseizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ABAT (Source: Uniprot.org, NCBI)

Gene Name

ABAT

Full Name

4-aminobutyrate aminotransferase, mitochondrial

Weight

56.439kDa

Superfamily

class-III pyridoxal-phosphate-dependent aminotransferase family

Alternative Names

(S)-3-amino-2-methylpropionate transaminase; 4-aminobutyrate aminotransferase; 4-aminobutyrate aminotransferase, mitochondrial; 4-aminobutyrate transaminase; EC 2.6.1.19; EC 2.6.1.22; FLJ17813; FLJ30272; G; GABA aminotransferase; GABA transaminase; GABA transferase; GABA-AT; GABA-T; GABATNPD009; Gamma-amino-N-butyrate transaminase; hCG1984265; L-AIBAT ABAT GABA-AT, GABAT, NPD009 4-aminobutyrate aminotransferase 4-aminobutyrate aminotransferase, mitochondrial|(S)-3-amino-2-methylpropionate transaminase|4-aminobutyrate transaminase|GABA aminotransferase|GABA transaminase|GABA transferase|gamma-amino-N-butyrate transaminase|gamma-aminobutyrate aminotransferase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ABAT, check out the ABAT Infographic

ABAT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ABAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for PB10019

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-ABAT Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-ABAT Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-ABAT Antibody Picoband™

Question

Is this PB10019 anti-ABAT antibody reactive to the isotypes of ABAT?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

The immunogen of PB10019 anti-ABAT antibody is E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-09

Question

I have a question about product PB10019, anti-ABAT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10019 anti-ABAT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ABAT antibody PB10019. Let me know if you need anything else.

C. Anderson

Verified customer

Asked: 2016-12-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-12-22

Question

Would anti-ABAT antibody PB10019 work on pig WB with pancreatic islet?

A. Taylor

Verified customer

Asked: 2016-05-12

Answer

Our lab technicians have not tested anti-ABAT antibody PB10019 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ABAT antibody PB10019 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig pancreatic islet in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-05-12

Question

We are currently using anti-ABAT antibody PB10019 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

S. Carter

Verified customer

Asked: 2015-09-18

Answer

The anti-ABAT antibody (PB10019) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-09-18

Question

Would anti-ABAT antibody PB10019 work for WB with liver?

S. Patel

Verified customer

Asked: 2013-01-15

Answer

According to the expression profile of liver, ABAT is highly expressed in liver. So, it is likely that anti-ABAT antibody PB10019 will work for WB with liver.

Boster Scientific Support

Answered: 2013-01-15

Size

Conjugation

Total: $370

SKU:PB10019

Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
Eddy test
In stock
Order Product
PB10019
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.