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- Table of Contents
AGO2 · Western blot design guide
AGO2 Western Blot Planning Guide
Plan an AGO2 Western blot around the catalog-observed 97.2 kDa band, image-backed PB9978 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
AGO2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 97.2 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Breast | |
| Negative control | Smooth muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 97.2 kDa |
| ⓘ | Localization | Cytoplasm, P-body / Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Sample controls for AGO2 Western blot
🧪Use Breast as the first positive-control candidate and Smooth muscle as the HPA Not detected negative candidate.
Positive control: Breast (High)
Negative control: Smooth muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for AGO2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 1
What Is the Expected AGO2 Western Blot Band Size?
Use the product-observed 97.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 97.2 kDa | Matches the authoritative product WB observation. |
| 97.2 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected AGO2 appearancePlan around 97.2 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 97.2 kDa; use this as the primary experimental expectation. |
| Calculated mass | 97.2 kDa from UniProt Q9UKV8; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9978. |
| Situation | Likely cause | Next action |
|---|---|---|
| 97.2 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing AGO2 band
PB9978 · AGO2Observed 97.2 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated AGO2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9978 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced AGO2 Western Blot Tips
Deeper troubleshooting and optimisation questions for AGO2, answered from its protein features.
Which band should guide the blot?
Use 97.2 kDa, the observation attached to the authoritative PB9978 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 97.2 kDa expectation.
Which positive control should I start with?
Start with Breast, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Smooth muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for AGO2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9978 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC3570096, PMC6308973, PMC3859937.
Boster reagents
AGO2 Western Blot Reagents
Human/Mouse/Rat-reactive AGO2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Ago2/eIF2C2 Antibody Picoband®
Cat # PB9978
Reactivity: Human, Mouse, Rat
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9UKV8
- Human Protein Atlas — AGO2
- PB9978 product record
- PMC3570096 — T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.
- PMC6308973 — Control of hepatic gluconeogenesis by Argonaute2.
- PMC3859937 — Strong reduction of AGO2 expression in melanoma and cellular consequences.