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- Table of Contents
APEX1 Antibodies
AND APEX1 ELISA kits, APEX1 Proteins
Many biological assays use antibodies to detect APEX1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with APEX1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop APEX1 antibodies...
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APEX1 Infographic by Boster Bio
All APEX1 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # PA1494
100 μg/vial
Cat # PA1494
100 μg/vial
Anti-APE1/APEX1 Antibody Picoband®
1 Citations 1 Q&As
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # PB9128
100 μg/vial
Cat # PB9128
100 μg/vial
Human
Flow Cytometry, IHC, ICC, WB
$370
Cat # M00627
100 μg/vial
Cat # M00627
100 μg/vial
Human, Mouse, Rat
ELISA
$480
Cat # EKC1700
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1700
1 kit, containing one 96-well plate and all necessary reagents
Dog, Human, Monkey, Mouse, Rat
IHC, WB
$523
Cat # M00627-1
100 µl
Cat # M00627-1
100 µl
APEX1 Overview
Facts about DNA-(apurinic or apyrimidinic site) lyase.
DNA-(apurinic or apyrimidinic site) lyase (APEX1)
Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors.
Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease from the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the harm, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.
Gene Information
| Human | |
|---|---|
| Gene Name: | APEX1 |
| Uniprot: | P27695 |
| Entrez: | 328 |
Family
| Belongs to: |
|---|
| DNA repair enzymes AP/ExoA family |
Alternative Names
AP endonuclease 1; APE; APE1; APE-1; APEAPEX nuclease (multifunctional DNA repair enzyme); APENAP endonuclease class I; APEX deoxyribonuclease (apurinic or apyrimidinic); APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease; APEX1; Apurinic-apyrimidinic endonuclease 1; APXAP lyase; DNA-(apurinic or apyrimidinic site) lyase; EC 3.1; EC 4.2.99.18; HAP1apurinic/apyrimidinic (abasic) endonuclease; multifunctional DNA repair enzyme; protein REF-1; redox factor 1; Redox factor-1; REF1 apurinic/apyrimidinic exonuclease; REF-1
Molecular Weight
Mass (kDA):
35.555 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 14q11.2 |
| Sequence: | 14; NC_000014.9 (20455226..20457767) |
Subcellular Localizations
Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimula
Diseases
- Malignant Neoplasms
- Neoplasms
- Carcinoma
- Malignant Neoplasm Of Lung
- Lung Neoplasms
- Carcinogenesis
- Adenocarcinoma
- Neoplasm Metastasis
- Leukemia
- Inflammation
- Mammary Neoplasms
- Cell Transformation, Neoplastic
- Hypoxia
Interacting Proteins
- EP300
- Rnf4
- SIRT1
Pathways
- Dna Repair
- Localization
- Cell Death
- Pathogenesis
- Cell Cycle
- Cell Proliferation
- Cell Growth
- Aging
- Response To Oxidative Stress
- Base-excision Repair
- Cell Killing
- Dna Replication
- Hypersensitivity
PTMs
- Cleavage
- Phosphorylation
- Reduction
- Oxidation
- Acetylation
- Deamination
- Ubiquitination
- Methylation
- Glutathionylation
- Demethylation
Research Areas
- Autophagy
- Base Excision Repair
- Cancer
- Core ESC-Like Genes
- DNA Repair
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/APEX1
bosterbio.com/bosterbio-gene-info-cards/APEX1
References
PMID: 1719477 by Robson C.N., et al. Isolation of cDNA clones encoding a human apurinic/apyrimidinic endonuclease that corrects DNA repair and mutagenesis defects in E. coli xth (exonuclease III) mutants.
PMID: 1722334 by Demple B., et al. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.