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- Table of Contents
APEX1 antibody (PB9128) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of U937 cells using anti-APEX1 antibody (PB9128).
Overlay histogram showing U937 cells stained with PB9128 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APEX1 Antibody (PB9128,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U937 cells stained with PB9128 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APEX1 Antibody (PB9128,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot or IHC for APEX1? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-APE1/APEX1 Antibody Picoband®
APEX1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
APEX1 antibody was positively detected by WB in:
human K562 whole cell, human HepG2 whole cell, human Raji whole cell, human A431 whole cell, human A549 whole cell, human MCF-7 whole cell, human RT4 whole cell, human U87 whole cell, human K562 whole cell, human HepG2 whole cell, human Raji whole cell, human RT4 whole cell, rat C6 whole cell, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
APEX1 antibody was positively detected by IHC in:
human lung cancer tissue, mouse brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
APEX1 antibody was positively detected by ICC/IF in:
U20S cell
APEX1 antibody was positively detected by IF in:
human colon cancer tissue
APEX1 antibody was positively detected by FC in:
U937 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2 μg/ml
Human
Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-APE1/APEX1 Antibody Picoband®
View all APEX1 Antibodies
View all APEX1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
35.6 kDa
Background of APEX1
APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Cite This Product
Anti-APE1/APEX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9128)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
95%
High Homology
Rabbit
95%
High Homology
Mouse
94%
High Homology
Cow
94%
High Homology
Rat
93%
High Homology
Sheep
93%
High Homology
Zebrafish
66%
Low homology
Drosophila
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for APEX1 antibody
Immunohistochemistry (IHC) protocol for APEX1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for APEX1 antibody
Immunofluorescence (IF) protocol for APEX1 antibody
Flow Cytometry (FC) protocol for APEX1 antibody
Publications
Application:
Species:
Epigenetic silencing of TET1 mediated hydroxymethylation of base excision repair pathway during lung carcinogenesis
Published: 2020-10-15
Journal: ENVIRONMENTAL POLLUTION
Application: WB
Impact Factor: 6.79
Species: Human
PMID: 33120142
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Product Promise
We promise Anti-APE1/APEX1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9128
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026