ATG13 Antibodies

Autophagy-related protein 13 (ATG13)

Autophagy factor needed for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway which regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of this ATG13-ULK1- RB1CC1 complicated.

Throughout its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. .

Gene Information

Human
Gene Name:	ATG13
Uniprot:	O75143
Entrez:	9776

Family

Belongs to:
ATG13 family

Alternative Names

autophagy-related protein 13; autophagy related 13; KIAA0652

Molecular Weight

Mass (kDA):
56.572 kDA

Genomic Context

Human
Location:	11p11.2
Sequence:	11; NC_000011.10 (46617276..46676019)

Subcellular Localizations

Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.

Diseases

• Tissue Adhesions
• Neoplasms
• Sclerosis
• Waldenstrom Macroglobulinemia
• Nerve Degeneration
• Muscular Atrophy
• Monoclonal Gammapathies
• Monoclonal Gammopathy Of Undetermined Significance
• Feline Infectious Peritonitis And Pleuritis

• Multiple Myeloma
• Malignant Neoplasms
• Tuberous Sclerosis

Interacting Proteins

• ATG101
• GABARAP
• GABARAPL1
• GABARAPL2
• MAP1LC3B

• MAP1LC3C
• RB1CC1
• ULK1

Pathways

• Autophagy
• Macroautophagy
• Transport
• Localization
• Response To Nutrient
• Catabolic Process
• Cell Growth
• Cell Death
• Cvt Pathway
• Aging
• Regulation Of Autophagy
• Oogenesis
• Cell Proliferation

• Hyperphosphorylation
• Cellular Homeostasis
• Biosynthetic Process
• Translation
• Aggrephagy
• Secretory Pathway
• Vesicle Transport

PTMs

• Phosphorylation

• Dephosphorylation

• Cleavage

Research Areas

• Phospho-Specific

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATG13

Best Uses Of The ATG13 Marker

In this article, we will look at the Anti-Autophagy-related protein (ATG13) marker and its applications in various research studies. The Reagents used by Boster Bio including ATG13 Label, a Molecular Label will be covered. Learn about the reagents you can use in Boster Bio as well. Below are a few of the most commonly used applications for ATG13.

Anti-Autophagy-related protein 13 (ATG13)

Autophagy is an important process in eukaryotes. It is activated when there is starvation. Autophagy is essential to control the quality of proteins in intracellular cells and preimplantation development. It also assists in eliminate intracellular pathogens as well as certain types of cell deaths. Numerous processes involve protein, including tumor suppression, antigen presentations, and autophagosome creation.

Researchers have identified anti-Autophagy-related protein 13 (AGG13) in the Boster Bio lab. Researchers have found that the protein is involved in the suppression of autophagy. They have found that ATG13 dephosphorylates NF-kappaB, that regulates autophagy. p53, which modulates necrosis.

GFP-Atg13 has been proven to colocalize with a marker protein named Atg16L1 in isolation membranes. Mizushima and colleagues published research that further confirms the interaction between ULK1 and ATG13. The GFP-ATG13 fusion proteins are available at a price of $3250. InVivo Biosystems offers a variety of Cas9 proteins Nuclease Vectors/Viruses and Cell Lines. This protein can be obtained from Control Lentiviruses or Lentivectors which contain luciferase and GFP.

Autophagy inhibition is a promising treatment strategy and many agents are currently in the early stages of clinical trials. If they are successful, autophagy inhibition could help patients with a wide range of ailments. By inhibiting this process, patients can overcome resistance to drugs and suffer more apoptotic cells death. This is a promising area for research. However it is important to recognize that there are many open questions.

The BbATG13 homologs to Atg13 show a significant degree in the degree of similarity between the two proteins. They are more closely related to Cordyceps militaris and Metarhizium species. BbATG13 also contains two amino acids which are phosphorylated within Aspergillus species differently. Additionally, it is homologous to Neurospora crassa Atg13.

Subcloning the Atg13 cDNA into pCold I was done and expressed in Escherichia coli. The fusion proteins were purified by GST-Atg13. The antibody binds autophagy-related proteins 13 (ATG13) in a spectral manner.

Autophagy is a degrading system that produces amino acids in cells and assists them to adapt for starvation. mTOR has been linked with cancer and has been identified as a negative regulator of autophagy. The autophagic isolation membrane is home to the protein Atg13. It is required for autophagosome development. The complex created by ULK1 - ATG13 FIP200 is not affected by nutrient conditions.

The Autophagy pathway has been linked to a range of physiological processes in filamentous fungi. In B. bassiana autophagy takes place throughout the life cycle. There are multiple stages of fungal development, and virulence are affected by the ATG1 gene. The Atg1 Kinase also plays a large role in the phosphoproteome as well as the proteome.

Applications

The ATG13 gene is a vital regulator of autophagy. Autophagy is triggered when there is a shortage of nutrients. It is controlled by the mTOR kinase that activates ATG13, and ULK1. ATG13 binds to FIP200 and facilitates phosphorylation of ULK1. The knockdown of ATG13 inhibits autophagosome formation.

The pGEX-6P-2 expression vector was used to express the ATG13 gene, ULK1 and ULK2 in BL21(DE3) cell lines. The cells were treated with 0.1 mM isopropyl-1-thio-b-galtopyranoside and the fusion proteins were purified using glutathione-Sepharose 4B beads.

The ATG8 family proteins are specialized. LC3C is an example of a protein that plays a role in autophagy of bacteria-infected cells. The LC3C molecule interacts to the protein NDP52. Two groups independently developed probes to determine the best autophagy probe. Lee's research team found that the LIR motif in p62 wasn't sufficient to localize. They therefore merged the LIR motif of p62 with the short-form phosphodiesterase 4. The result was HyD/GFP-LIR constructs.

The cross-correlation between ULK1 and Atg13 demonstrated that the two proteins colocalized within 100 nanometers. There were also colocalized ATG13 clusters that extended past 200 millimeters. These error bars correspond to SEM of seven starved cells versus the ten cells fed. This suggests that Atg13 is a reliable marker for cancer detection. It has many applications.

reagents used in Boster Bio

For more than 25 years, Boster Bio has focused on the development of high-quality immunological Reagents. Their ELISA kits as well as research antibodies and ELISA kits can detect biomarkers in these areas including neurodevelopment, cancer inflammation, and neurosciences. Boster's kits are trusted by more than 10,000 scientists across the world. Boster's kits also come with the Boster Quality Guarantee, which means you can be confident that they'll perform as they claim to.

Boster Bio's Picokine ELISA Kit can be utilized to quantify and detect the presence of haptens in tissue sections. IHC utilizes enzymes to catalyze the binding between an antibody and an antigen. Since the antibodies bind to the antigens, the reaction creates the color. IHC is used extensively in clinical and research laboratories. The quality of the specimen is crucial to the success of the antigen presentation.