Anti-Autophagy-related protein 13 ATG13 Antibody

Product Name

Anti-Autophagy-related protein 13 ATG13 Antibody

View all ATG13 Antibodies

SKU/Catalog Number

A02874

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Autophagy-related protein 13 ATG13 Antibody (Catalog # A02874). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Autophagy-related protein 13 ATG13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02874)

Host

Rabbit

Contents

ATG13 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG13 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATG13. The immunogen is located within amino acids 450 - 500 of ATG13.

Cross-reactivity

Multiple isoforms of ATG13 are known to exist.

Reactive Species

A02874 is reactive to ATG13 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

56.6 kDa

Background of ATG13

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02874 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG13 antibody can be used for detection of ATG13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG13

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