ATP synthase-coupling factor 6, mitochondrial (ATP5PF)

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or intricate V) generates ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transfer complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - comprising the extramembraneous catalytic core and F(0) - comprising the membrane proton channel, connected together by a central stalk and a peripheral stalk.

During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled by means of a rotary mechanism of the central stalk subunits to proton translocation. Part of the intricate F(0) domain and the peripheric stalk, which functions as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.