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- Table of Contents
ATP5PF antibody (A32371-1) performs well in IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Fluorescent confocal image of U251 cell stained with ATP5J Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ATP5J immunoreactivity is localized to Mitochondria significantly.
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Anti-ATP5J Antibody (Center)
ATP5PF antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-ATP5J Antibody (Center)
View all ATP5PF Antibodies
View all ATP5PF Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.
Calculated Molecular Weight
12.6 kDa
Background of ATP5PF
Mitochondrial ATP synthase catalyzes ATP synthesis,
utilizing an electrochemical gradient of protons across the inner
membrane during oxidative phosphorylation. It is composed of two
linked multi-subunit complexes: the soluble catalytic core, F1, and
the membrane-spanning component, Fo, which comprises the proton
channel. The F1 complex consists of 5 different subunits (alpha,
beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3
beta, and a single representative of the other 3. The Fo seems to
have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene
encodes the F6 subunit of the Fo complex, required for F1 and Fo
interactions. Alternatively spliced transcript variants encoding
different isoforms have been identified for this gene. A pseudogene
exists on chromosome Yp11.
Cite This Product
Anti-ATP5J Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32371-1)
Publications
Application:
Species:
Impacts of Dietary Pleurotus eryngii Polysaccharide on Nutrient Digestion, Metabolism, and Immune Response of the Small Intestine and Colon—An iTRAQ-Based Proteomic Analysis
Published: 2018-02-05
Journal: PROTEOMICS
Application: WB
Impact Factor: 3.53
Species: Mouse
PMID: 29405577
Estrogen Represses Hepatocellular Carcinoma (HCC) Growth via Inhibiting Alternative Activation of Tumor-associated Macrophages (TAMs) *
Published: 2012-08-20
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB
Impact Factor: 4.77
Species: Mouse
PMID: 22908233
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Product Promise
We promise Anti-ATP5J Antibody (Center) performs excellently in relevant applications for IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A32371-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026