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CD2AP / CD2-associated protein · IHC design guide
Design Immunohistochemistry for CD2AP
Plan chromogenic CD2AP IHC on paraffin sections. Use cytoplasmic staining in most cells and strong staining in glomerular cells as benchmarks (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
CD2AP Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic in most tissue cells (HPA tissue IHC) | |
| Staining pattern | Most cells show cytoplasmic staining (HPA tissue IHC) | |
| Antigen retrieval | Citrate pH 6 HIER, heat-mediated (datasheet M01756) | |
| Positive control | Bone marrow+4 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across sections (standard IHC practice; not target-specific) | |
| Caveat | Glomerular cells stain strongly; adipocytes are undetected (HPA tissue IHC) | |
| Regulation | No specific staining regulator reported (UniProt) | |
| Isoform / epitope | No reported isoforms or chain processing (UniProt) |
Section 1
Recommended CD2AP IHC & IF Protocols
Start with the catalog antibody’s IHC-P protocol (datasheet M01756), then compare published CD2AP staining in brain sections (PMC8713526) and a gastric cancer tissue microarray (PMC7078920).
| Sample | Paraffin-embedded human colon cancer tissue; fixative not specified (datasheet M01756) |
| Fixation | Image fixative and duration unreported (datasheet M01756); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: Citrate pH 6, 20 min (datasheet M01756) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet M01756) |
| Primary antibody | Mouse monoclonal (clone 5F8) anti-CD2AP, 0.5-1μg/ml (datasheet M01756) |
| Primary incubation | Overnight at 4 °C (datasheet M01756) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet M01756) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | CD2AP-positive staining in hematopoietic cells of bone marrow (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression in most cells. No signal in the no-primary control. |
💡Decision noteTry heat-mediated citrate retrieval at pH 6 first (datasheet M01756); the gastric cancer protocol also uses citrate buffer but does not specify its pH or heating conditions (PMC7078920).
Section 2
What Is the Expected CD2AP Staining Pattern?
CD2AP is expected mainly in the cytoplasm of tissue cells (HPA: Enhanced tissue IHC; cytoplasmic expression in most cells). Strong staining is documented in kidney glomerular cells, several glandular epithelia and bone marrow hematopoietic cells (HPA: High in these cell populations). Its association with the cytoskeleton and cell junctions can add peripheral detail (UniProt Q9Y5K6: subcellular location); CD2AP has no transmembrane segment (UniProt Q9Y5K6: topology).
| Clear cytoplasmic staining in glomerular cells, with signal in breast or colon glandular cells. | This fits high expression in those cell populations (HPA: kidney, breast and colon tissue IHC). Score the stained cells and compartment, since neighboring structures need not share the same intensity. A glomerular signal alone does not identify individual podocytes: slit diaphragm localization is annotated by similarity (UniProt Q9Y5K6). |
| Predominantly nuclear staining, with little convincing cytoplasmic signal in known positive cells. | Treat a nuclear only pattern as suspect: HPA describes cytoplasmic tissue staining, while UniProt places CD2AP at the cytoskeleton, ruffles and junctions (HPA: tissue profile; UniProt Q9Y5K6: subcellular location). Review morphology, counterstain and controls before scoring it as CD2AP. |
| Strong staining in adipocytes, smooth muscle cells or ovarian stromal cells. | Those populations are reported as not detected (HPA: adipose tissue, smooth muscle and ovary tissue IHC). Check whether the signal follows the named cells rather than nearby positive cells. Cross reactivity or endogenous detection activity is possible; this pattern alone cannot establish which cause applies. |
| Uniform color over cells, extracellular space or the whole section, obscuring cell boundaries. | Diffuse background cannot be interpreted as the cell associated pattern reported for CD2AP (HPA: cytoplasmic expression in most cells). In chromogenic IHC, compare a no primary control and inspect tissue edges or deposits; background can arise from detection chemistry or excess nonspecific binding. |
| No convincing signal in glomerular cells or a documented strongly stained glandular epithelium. | An absent signal in these reference populations warrants a technical check (HPA: High in kidney glomerular cells and breast or colon glandular cells). Confirm that the relevant cells are present, then check the run controls and assay settings. A blank section alone cannot establish biological absence. |
💡Expected CD2AP appearanceCall a section positive when discrete cytoplasmic staining is strong in a documented positive population, such as kidney glomerular cells or breast glandular cells (HPA: High); isolated nuclear color or equally strong staining of HPA negative cell populations is suspect (HPA: tissue profile).
| Cell population and tissue | CD2AP has broad tissue expression (UniProt Q9Y5K6: tissue specificity; HPA: low RNA tissue specificity), but IHC levels differ by cell population. HPA reports High in kidney glomerular cells and several glandular epithelia, Low in selected muscle and glandular cells, and Not detected in adipocytes (HPA: tissue IHC). Choose the comparison cells accordingly. |
| Compartment and topology | The tissue IHC reference is mainly cytoplasmic (HPA: tissue profile). UniProt also places CD2AP at cytoskeletal structures, membrane ruffles and cell junctions, without a transmembrane segment (UniProt Q9Y5K6: subcellular location; topology). Peripheral staining can therefore fit the record, but a purely nuclear tissue pattern lacks support from these sources. |
| Cell division | UniProt reports concentration near midzone microtubules during late anaphase and telophase, and at the midbody in late telophase (UniProt Q9Y5K6: subcellular location). Interpret a small mitotic focus in its cellular context; this annotation does not establish that such detail will be resolved in every paraffin IHC section. |
| Evidence for the staining pattern | HPA rates tissue staining reliability Enhanced, citing consistency with RNA expression, and lists Enhanced IHC validation for HPA003267, HPA003326 and CAB004352 (HPA: tissue IHC; antibody validation). These ratings support the reference pattern; they do not verify an individual run, specimen or unlisted antibody. |
| Situation | Likely cause | Next action |
|---|---|---|
| The positive control is blank. | The expected cells may be absent from the section, or the IHC run may have failed; HPA reports High staining in kidney glomerular cells and breast glandular cells (HPA: tissue IHC). | Locate the reference cells on the counterstained section. Check the run control, primary antibody application, retrieval record and detection steps using the validated assay instructions. The supplied sources give no CD2AP specific retrieval condition or fixation sensitivity. |
| Signal is weak only in a low expression tissue. | A weak result may match the reference range: HPA lists Low in parathyroid glandular cells and cardiomyocytes (HPA: tissue IHC). | Compare a High reference population in the same run before adjusting the assay (HPA: High in kidney glomerular cells). Record the cell type scored; low signal alone does not show that retrieval or detection failed. |
| Unexpected nuclear staining dominates. | The dominant compartment differs from HPA tissue IHC and UniProt localization (HPA: cytoplasmic profile; UniProt Q9Y5K6: cytoskeleton and junctions). | Recheck the counterstain, focus and cell boundaries. Compare the no primary control and, where available, an independently validated IHC antibody (HPA: HPA003267, HPA003326 and CAB004352, IHC Enhanced). |
| Adipocytes or smooth muscle cells stain strongly. | Those cell types are listed as Not detected (HPA: adipose tissue and smooth muscle tissue IHC); cross reactivity or endogenous detection activity may explain the discrepancy. | Confirm cell identity and compare the no primary control. If color persists without primary antibody, investigate detection background; if it depends on primary antibody, compare an independent IHC validated antibody (HPA: antibody validation). |
| The section has widespread chromogenic haze. | Widespread color obscures the cell associated pattern reported by HPA (HPA: cytoplasmic tissue profile). Its precise cause cannot be inferred from appearance alone. | Compare a no primary control, inspect background outside cells, and review blocking, washes and detection timing as general chromogenic IHC checks. Score CD2AP only where cellular boundaries and a supported compartment remain clear. |
| Can the tissue IHC pattern be used to judge IF/ICC images? | The assays have different reference observations: HPA tissue IHC is mainly cytoplasmic, while HPA ICC-IF supports plasma membrane localization and marks centriolar satellites uncertain (HPA: tissue IHC; subcellular summary). | For IF/ICC, assess membrane signal against the HPA subcellular reference and treat satellite puncta cautiously (HPA: subcellular summary). Do not require the same apparent distribution at chromogenic section resolution; use the separate IF/ICC guide for assay setup. |
Experiment evidence
IHC & IF images showing CD2AP staining
M01756 · IHCIHC analysis of CD2AP using anti-CD2AP antibody (M01756). CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediat
M01756 · IHCIHC analysis of CD2AP using anti-CD2AP antibody (M01756). CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediat
M01756 · IFICC analysis of CD2AP using anti-CD2AP antibody (M01756). CD2AP was detected in immunocytochemical section of A431 cell. Enzyme antigen retr
A01756-2 · IHCIHC analysis of CD2AP using anti-CD2AP antibody (A01756-2). CD2AP was detected in a paraffin-embedded section of mouse kidney tissue. Heat m
A01756-2 · IHCIHC analysis of CD2AP using anti-CD2AP antibody (A01756-2). CD2AP was detected in a paraffin-embedded section of rat kidney tissue. Heat med
A01756-2 · IFIF analysis of CD2AP using anti-CD2AP antibody (A01756-2). CD2AP was detected in an immunocytochemical section of Hela cells. Enzyme antigen
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000198087-CD2AP/tissue/cerebral+cortex#imid_1212752
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000198087-CD2AP/tissue/colon#imid_1212670
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000198087-CD2AP/tissue/liver#imid_1212679
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000198087-CD2AP/tissue/kidney#imid_1212672
HPA · ICC-IF RT-4Human Protein Atlas immunofluorescence, RT-4, antibody HPA003267; green = CD2AP, blue = nucleus, red = microtubules.
HPA · ICC-IF RT-4Human Protein Atlas immunofluorescence, RT-4, antibody HPA003267; green = CD2AP, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for CD2AP IHC & IF
🧪Run colon first and require staining in its glandular cells (HPA: High in colon glandular cells). Use adipose tissue adipocytes as the negative tissue (HPA: Not detected in adipocytes); assess non-glandular cells on the colon slide for background, without assuming they are CD2AP-negative (UniProt Q9Y5K6: widely expressed).
Positive control tissue: Bone marrow (Hematopoietic cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show CD2AP in RT-4, U-251MG, U2OS, A-431, NIH 3T3, with annotated localisation: Plasma membrane (supported) (HPA subcellular).
Technical controls: Include no-primary (secondary-only) and concentration-matched mouse IgG isotype controls for the mouse catalog antibody, plus CD2AP-knockout material or a validated peptide-block control (caption: mouse anti-CD2AP M01756; standard IHC controls). Quench endogenous peroxidase and assess endogenous biotin before using the caption’s biotin-based DAB detection (caption: biotinylated secondary, SABC and DAB; standard IHC practice).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected M01756 paraffin-section caption does not state the fixative (caption: fixative not stated). The caption documents citrate retrieval at pH 6 for 20 minutes, but does not establish whether retrieval is required (caption: heat-mediated citrate retrieval). Neither a frozen-section or IF advantage nor a colon-specific artefact is established by the supplied evidence; evaluate background in the chosen tissue (standard IHC practice).
HPA tissue IHC evidence for CD2AP
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — High consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bone marrow | Hematopoietic cells | High | Protein (IHC) | HPA → |
| Breast | Glandular cells | High | Protein (IHC) | HPA → |
| Colon | Glandular cells | High | Protein (IHC) | HPA → |
| Duodenum | Glandular cells | High | Protein (IHC) | HPA → |
| Endometrium | Glandular cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | Glial cells | Not detected | Protein (IHC) | HPA → |
| Hippocampus | Glial cells | Not detected | Protein (IHC) | HPA → |
| Ovary | Ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | Smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CD2AP IHC Tips
Troubleshoot CD2AP staining in paraffin sections by checking retrieval, cell type, subcellular pattern, and controls before interpreting chromogenic signal.
Which antigen retrieval should I use for CD2AP paraffin sections?
Start with heat-mediated citrate buffer at pH 6 for 20 minutes (datasheet M01756). This condition produced CD2AP staining in a paraffin-embedded human colon cancer section using the catalog antibody (datasheet M01756). Keep heating, cooling, and subsequent staining conditions consistent while comparing sections, because retrieval differences can change chromogenic signal (standard IHC practice). If staining remains weak, assess a second retrieval condition on matched sections alongside the documented citrate condition, rather than assigning the change to CD2AP biology (standard IHC practice). Include a known positive tissue compartment and a no-primary control in that comparison (HPA tissue IHC; standard IHC practice).
How should I troubleshoot fixation-related variation in CD2AP IHC?
The selected paraffin-section caption does not state a fixative, so target-specific fixation sensitivity for CD2AP is unknown (datasheet M01756). Record the actual fixative and fixation time for each specimen, then compare sections processed with the same retrieval and detection conditions (standard IHC practice). If signal varies across a batch, examine whether the difference tracks fixation history, section quality, or staining run before interpreting it as altered expression (standard IHC practice). Use matched positive tissue and no-primary controls to assess each run (standard IHC practice). Do not infer a preferred fixation condition from CD2AP tissue distribution or its cytoskeletal association (HPA tissue IHC; UniProt Q9Y5K6).
Where should convincing CD2AP staining appear in tissue sections?
Expect predominantly cytoplasmic staining across many cell types in tissue IHC (HPA tissue IHC). CD2AP also associates with the cytoskeleton and cell junctions, and is reported at podocyte slit diaphragms, so examine cellular boundaries in the appropriate tissue context (UniProt Q9Y5K6). High staining in colon glandular cells and kidney glomerular cells provides useful compartment-level references (HPA tissue IHC). Score the relevant cells separately from neighboring compartments, using morphology and a counterstain to identify them (standard IHC practice). A purely nuclear or diffuse extracellular deposit is inconsistent with the reported CD2AP locations and warrants a review of background and controls (UniProt Q9Y5K6; standard IHC practice).
Could isoforms or epitope accessibility explain discordant CD2AP staining?
The supplied record lists one CD2AP chain, residues 1–639, and zero isoforms; it does not support assigning discordant staining to a named splice variant (UniProt Q9Y5K6). CD2AP has three annotated SH3 regions, including residues 108–167 and 269–330, and multiple phosphorylation sites (UniProt Q9Y5K6). An antibody epitope is not identified in the selected tissue-IHC caption, so its accessibility cannot be predicted from those features alone (datasheet M01756). Compare matched sections under the documented citrate pH 6 retrieval and a controlled alternative if signal is weak (datasheet M01756; standard IHC practice). Interpret differences only after checking cell identity, staining controls, and section quality (standard IHC practice).
How can IF help assess an ambiguous CD2AP IHC pattern?
Use IF as a separate localization check when chromogenic CD2AP staining leaves cell boundaries or cellular identity uncertain (standard IF practice). Multiplex CD2AP with a marker for the expected cell population, such as an independently validated glandular-cell or glomerular-cell marker selected for the specimen (HPA tissue IHC; standard IF practice). Choose spectrally distinct fluorophores after inspecting tissue autofluorescence, and include single-label controls to assess channel bleed-through (standard IF practice). CD2AP lacks a transmembrane segment and is associated with cytoplasm and cytoskeleton, so validate permeabilisation for access to its intracellular epitope (UniProt Q9Y5K6; standard IF practice). Compare IF and IHC by cell type and distribution without treating fluorescent intensity as equivalent to DAB intensity (standard IHC/IF practice).
What should I check when CD2AP DAB staining is widespread?
First distinguish plausible widespread cytoplasmic expression from nonspecific color: CD2AP is widely expressed, and tissue IHC reports cytoplasmic staining in most cells (UniProt Q9Y5K6; HPA tissue IHC). Examine no-primary and secondary-only controls, endogenous peroxidase blocking, and the distribution of precipitate before adjusting the primary antibody (standard IHC practice). The selected caption used 10% goat serum blocking, 1 µg/mL primary antibody overnight at 4°C, and biotin-based DAB detection (datasheet M01756). Because that detection system uses biotin, assess tissue-dependent endogenous biotin signal if controls show staining (datasheet M01756; standard IHC practice). Compare staining in expected positive cells with nearby cells reported as low or undetected, where the section permits (HPA tissue IHC).
How should I quantify CD2AP staining across paraffin sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and compartment before scoring: CD2AP tissue IHC is mainly cytoplasmic, with high reported staining in colon glandular and kidney glomerular cells (HPA tissue IHC). For a defined cell population, record the percentage of positive cells and an H-score based on intensity categories; use positive-cell density per mm² when cell counts per tissue area answer the study question (standard IHC practice). Normalize counts to viable tissue area or the number of eligible cells in the same compartment, and report excluded regions (standard IHC practice). Apply the same staining run, imaging settings, thresholds, and scoring rules across comparison groups (standard IHC practice). Keep cell populations separate because their baseline CD2AP staining differs (HPA tissue IHC).
How do I distinguish true CD2AP signal from artefact?
A convincing result follows cellular morphology and the expected cytoplasmic distribution, with attention to junctional context where it can be resolved (HPA tissue IHC; UniProt Q9Y5K6). Check whether the labeled cells fit the specimen: colon glandular cells and kidney glomerular cells show high tissue-IHC staining, while adipocytes and smooth muscle cells are reported as undetected (HPA tissue IHC). Treat isolated nuclear color, tissue-edge accentuation, necrotic deposits, and signal in no-primary controls as reasons to investigate artefact (UniProt Q9Y5K6; standard IHC practice). Review peroxidase blocking and biotin-related background because the selected caption used biotin-based DAB detection (datasheet M01756; standard IHC practice). Interpret staining only after matching the observed pattern to cell identity and control performance (standard IHC practice).
Boster reagents
Best CD2AP / CD2-associated protein IHC Antibodies
CD2AP IHC is illustrated in human colon cancer (M01756 IHC caption) and mouse kidney (A01756-2 IHC caption); IF is illustrated in HeLa cells (A01756-2 IF caption).
Real IHC data
Anti-CD2AP Antibody ® (monoclonal, 5F8)
Cat # M01756
Real IHC data
Anti-CD2AP Antibody ®
Cat # A01756-2
M01756 has paraffin-section IHC images from human colon cancer, placenta, and mammary cancer (M01756 IHC captions). A01756-2 has paraffin-section IHC images from mouse and rat kidney and an IF image from HeLa cells (A01756-2 IHC/IF captions).
Which to pick: For human tissue IHC, choose M01756, a mouse monoclonal clone 5F8 with a human paraffin-section image using citrate pH 6 retrieval (catalog: M01756 host/clone; M01756 IHC caption). For IF/ICC, choose A01756-2, which has a HeLa IF image and lists both applications (A01756-2 IF caption; catalog: A01756-2 applications). For cross-species tissue IHC, choose A01756-2: its paraffin-section images show mouse and rat kidney with EDTA pH 8 retrieval, and its listed reactivity includes human, mouse, and rat; the fixative is unreported in both SKUs’ IHC captions (A01756-2 IHC captions; catalog: A01756-2 reactivity; M01756 IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q9Y5K6 (CD2AP_HUMAN, CD2-associated protein).
- Human Protein Atlas. CD2AP tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. CD2AP subcellular location (ICC-IF): Mainly localized to the plasma membrane. In addition localized to the centriolar satellites..
- Human Protein Atlas. CD2AP antibody validation summary (3 antibodies).
- Shensu IV maintains the integrity of the glomerular filtration barrier and exerts renal protective effects by regulating endogenous hydrogen sulfide levels. Frontiers in pharmacology 2024 — PMC11667557.
- Association of CD2AP neuronal deposits with Braak neurofibrillary stage in Alzheimer's disease. Brain pathology (Zurich, Switzerland) 2022 — PMC8713526.
- CD2AP inhibits metastasis in gastric cancer by promoting cellular adhesion and cytoskeleton assembly. Molecular carcinogenesis 2020 — PMC7078920.
- Tangzhiqing Granules Alleviate Podocyte Epithelial-Mesenchymal Transition in Kidney of Diabetic Rats. Evidence-based complementary and alternative medicine : eCAM 2017 — PMC5259601.
- PubMed PMID:10339567 — UniProt-cited evidence.
- PubMed PMID:14574404 — UniProt-cited evidence.
- PubMed PMID:15489334 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for CD2AP (CD2-associated protein). Expected localisation Cytoplasmic in most tissue cells (HPA tissue IHC). Antigen retrieval: Citrate pH 6 HIER, heat-mediated (datasheet M01756). Validated IHC antibody: M01756 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Bone marrow (Hematopoietic cells); negative Adipose tissue. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human colon cancer tissue; fixative not specified (datasheet M01756); 2. Fixation: Image fixative and duration unreported (datasheet M01756); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: Citrate pH 6 , 20 min (datasheet M01756); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet M01756); 8. Primary antibody: Mouse monoclonal (clone 5F8) anti-CD2AP, 0.5-1μg/ml (datasheet M01756); 9. Primary incubation: Overnight at 4 °C (datasheet M01756). Watch for: Fixation: Keep fixation consistent across sections (standard IHC practice; not target-specific); Caveat: Glomerular cells stain strongly; adipocytes are undetected (HPA tissue IHC); Isoform / epitope: No reported isoforms or chain processing (UniProt); Regulation: No specific staining regulator reported (UniProt). Evidence: UniProt Q9Y5K6, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the plasma membrane. In addition localized to…, Datasheet M01756, PMC8713526, PMC7078920. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/CD2AP/immunohistochemistry-ihc-guide.