COL4A1 Antibodies

Collagen alpha-1(IV) chain (COL4A1)

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. .

Gene Information

Human
Gene Name:	COL4A1
Uniprot:	P02462
Entrez:	1282

Family

Belongs to:
type IV collagen family

Alternative Names

alpha-1 chain; Arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2

Molecular Weight

Mass (kDA):
160.611 kDA

Genomic Context

Human
Location:	13q34
Sequence:	13; NC_000013.11 (110148963..110307157, complement)

Tissue Specificity

Highly expressed in placenta.

Subcellular Localizations

Secreted, extracellular space, extracellular matrix, basement membrane.

Diseases

• Hemorrhage
• Cerebral Hemorrhage
• Acquired Porencephaly
• Kidney Diseases
• Cerebrovascular Accident
• Leukoencephalopathies
• Fibrosis
• Aneurysm
• Tissue Adhesions
• Hereditary Nephritis
• Brain Diseases
• Intracranial Aneurysm
• Muscle Cramp

• Diabetic Nephropathy
• Congenital Abnormality
• Neoplasms
• Hemiplegia
• Malignant Neoplasms
• Vascular Diseases
• Carcinoma

Interacting Proteins

• COL4A2

Pathways

• Pathogenesis
• Cell Proliferation
• Secretion
• Angiogenesis
• Localization
• Excretion
• Cell Adhesion
• Tissue Development
• Reverse Transcription
• Translation
• Wound Healing
• Coagulation
• Cell Death

• Tissue Remodeling
• Rna Interference
• Transport
• Osteoclast Differentiation
• Cell Migration
• Developmental Process
• Osteoblast Differentiation

PTMs

• Cleavage

• Phosphorylation

• Oxidation

Research Areas

• Cell Biology
• Cellular Markers
• Extracellular Matrix
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/COL4A1

Boster Bio: Anti-Collagen IV/COL4A1 Marker

You've come across the Boster Bio Anti-Collagen IV/COL4A1 marker. This section provides information about the product and its applications. Boster Bio is committed to helping scientists make better and more informed decisions in the lab. To ensure high specificity and affinity, they validate their antibodies on different platforms using positive and negative samples. Boster also offers incentives to scientists who review their products early and does so with product credits.

Boster Bio: Anti-Collagen IV/COL4A1 Marker

The COL4A1 gene encodes type IV collagen. This flexible protein is crucial for the structure and function of a variety of tissues. The gene produces an alpha1(IV), chain, which combines to form the COL4A1 molecule. The alpha1-1-2 chains are linked to one another and form a huge complex protein network that is a major component of basement membranes.

Applications

The COL4A1 collagen gene is a unique type of collagen that is associated with cell expansion. The cells that are knocked down by this gene exhibit decreased viability and stop dividing. Genetic mutations in this gene can be associated with a range of clinical symptoms such as myopathy, porencephaly, and Walker-Warburg syndrome. Here are the most frequently occurring mutations and their clinical uses. In order to fully understand the functions of the COL4A1 gene in these clinical contexts, it is important to first know the function of the gene.

The COL4A1 gene is a structural element of basement membranes and their walls are composed of cells of different types. The tissue is referred to as a microenvironment. The cells that make up this microenvironment collaborate to regulate the elasticity of the blood vessels. The presence of tumors in the microenvironment can be used to identify its condition and its progression. COL1A1, COL4A1, and PDGFRB were shown to have negative correlations with tumor purity.

Utilizing lentiviral vectors to deliver shRNA, SKBR3 cells were infected with the COL4A1 or shRNA control. The cells were counted continuously for four days. Inhibition of COL4A1 decreased the levels of protein and mRNA. Although the effects of COL4A1 knockdown were minor but they did form colonies which suggests that the COL4A1 gene is a useful prognostic marker.

The COL4A1 gene is important for colony development and proliferation. In addition, it is involved in the formation of the blood-gas barrier. It could also be a therapeutic target. It is among the most upregulated genes involved in the formation of the avian blood-gas barrier. Additionally, the gene could help determine the extent of a specific cancer as well as its response to specific treatments.

Validation

GSEA analysis of transcriptome data from HCC patients was carried out to determine the clinical value and clinical efficacy of a COL4A1 mark. The expression of Type IV collagen was highly correlated with HCC, was increased in preneoplastic tissues, and was positively correlated with a shorter time-to-progression-free survival for HCC patients. This analysis revealed a novel interactive regulatory system for Type IV collagen. COL4A1/2 binds to integrin beta-2 and alpha-1, triggers sequential signal transduction, and enhances the cell cycle.

The ER is where the majority of the COL4A1 protein is. A mutation in COL4A1 can result in an increase in ER which could alter the response to unfolded proteins. A variation in COL4A2 could also result in the absence of or a decrease in COL4A2 secretory vesicles. This can help in identifying a COL4A1 related disorder.

A c.2317G–C transversion in COL4A1 resulted in a substitution between the gly730 and the arg1581. The mutation was not found in the control population and was associated with a wide range of cerebrovascular disorders. In a second study, Shah et al. discovered a mutation in one patient carrying the mutation that did not exist in 300 controls. The proband's first child born at 23 weeks gestation was a microcephalic child with cataracts that were congenital. He needed help with feeding and was diagnosed as having a stroke-related disease. The affected sibling had mild cataracts as well as a right-sided infantile hemiplegia.

Significant correlation was observed significant correlation was observed HCC patients and the COL4A1 -COL4A2 genes. In addition, the two markers share the same bidirectional promoter. This suggests that they can be useful in early HCC diagnosis. It is important to keep in mind that HCC and COL4A1 are closely related. Consequently, a COL4A1 gene test is the best candidate to assess a patient's risk for HCC.

The analysis revealed that the COL4A1 gene is mutated, that results in increased levels of intracellular glycine. Additionally, a patient carrying the mutation in the G646D gene displayed lower secretion as well as an increase in intracellular glycine level in cells. Additionally, immunolabeling revealed the presence of COL4A1/A2 heterotrimers within secretory vesicles.

Using in situ hybridization, Emanuel et al. Emanuel and. discovered that COL4A1 was assigned 13q34 which is very close to D13S3, a gene coding one of the subunits in the COL4A1 polypeptide chains. Further, Bowcock et al. Bowcock and others.

In addition to COL4A1 Two additional genes that were related to COL4A1 were studied. They had significantly lower levels of expression than COL4A2 and ACTN2 were also associated with HCC. This suggests that the genes associated with COL4A1 are more closely connected to HCC than PTK2.