COL4A1 Antibodies

AND COL4A1 ELISA kits, COL4A1 Proteins

Many biological assays use antibodies to detect COL4A1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with COL4A1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop COL4A1 antibodies...

We validate the specificity of these antibodies to COL4A1 by testing them on tissues known to express COL4A1 positively and negatively. Browse below to find the COL4A1 antibody that suites your experiment. We have 8 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for COL4A1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the COL4A1 Western Blot Guide

COL4A1 Infographic by Boster Bio

All COL4A1 Products

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Product

Figures

Specificity

Applications

Price

Anti-Collagen IV/COL4A1 Antibody Picoband®

24 Citations

IHC analysis of Collagen using anti-Collagen antibody (PB9099)

IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody (PB9099/M00063-2) COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues

Human

IF, IHC, WB

$370
Cat # PB9099
100 μg/vial

Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3)

1 Citations

IHC analysis of Collagen IV using anti-Collagen IV antibody (M01411)

Western blot analysis of Collagen IV using anti-Collagen IV antibody (M01411)

Human

IF, IHC, WB

$370
Cat # M01411
100 μg/vial

Anti-Collagen Type IV COL4A1 Antibody

1 Citations

Boster anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids

Immunohistochemistry results of Rabbit Anti-Collagen Type IV Antibody

Bovine, Human

ELISA, IP, IF, IHC, WB

$658
Cat # A01411
100ug

Anti-Collagen IV COL4A1 Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue

Immunofluorescence analysis of mouse-spleen tissue

Human, Mouse, Rat

IF, IHC

$380
Cat # M01411-1
100ul

Anti-COL4A1 (G46) Antibody

Western blot (WB) analysis of COL4A1 (G46) polyclonal antibody at 1:500 dilution
Lane1:MCF-7 cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat liver tissue lysate

Immunohistochemistry (IHC) analyzes of COL4A1 (G46) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC, WB

$405
Cat # A01411-1
100ul

Anti-Collagen IV Rabbit Monoclonal Antibody

Human appendix was stained with Anti-Collagen IV rabbit antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Collagen IV antibody

Human

ELISA, IP, IF, IHC, WB

$146
Cat # M01411-2
20μL, 40μL, 100μL, 200μL

Collagen IV Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells

Human, Mouse

ELISA

$480
Cat # EKC1128
1 kit, containing one 96-well plate and all necessary reagents

Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated

Bovine, Human

ELISA, IP, IHC, WB

$670
Cat # B02908
100ug

COL4A1 Overview

Facts about Collagen alpha-1(IV) chain.

COL4A1 3D structure. Source: alphafold

Collagen alpha-1(IV) chain (COL4A1)

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. .

Gene Information

Human
Gene Name:	COL4A1
Uniprot:	P02462
Entrez:	1282

Family

Belongs to:

type IV collagen family

Alternative Names

alpha-1 chain; Arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2

Molecular Weight

Mass (kDA):
160.611 kDA

Genomic Context


Human

Location:	13q34
Sequence:	13; NC_000013.11 (110148963..110307157, complement)

Tissue Specificity

Highly expressed in placenta.

Subcellular Localizations

Secreted, extracellular space, extracellular matrix, basement membrane.

Diseases

Hemorrhage
Cerebral Hemorrhage
Acquired Porencephaly
Kidney Diseases
Cerebrovascular Accident
Leukoencephalopathies
Fibrosis
Aneurysm
Tissue Adhesions
Hereditary Nephritis
Brain Diseases
Intracranial Aneurysm
Muscle Cramp

Diabetic Nephropathy
Congenital Abnormality
Neoplasms
Hemiplegia
Malignant Neoplasms
Vascular Diseases
Carcinoma

Interacting Proteins

COL4A2

Pathways

Pathogenesis
Cell Proliferation
Secretion
Angiogenesis
Localization
Excretion
Cell Adhesion
Tissue Development
Reverse Transcription
Translation
Wound Healing
Coagulation
Cell Death

Tissue Remodeling
Rna Interference
Transport
Osteoclast Differentiation
Cell Migration
Developmental Process
Osteoblast Differentiation

PTMs

Cleavage

Phosphorylation

Oxidation

Research Areas

Cell Biology
Cellular Markers
Extracellular Matrix
Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/COL4A1

References

PMID: 2701944 by Soininen R., et al. Structural organization of the gene for the alpha 1 chain of human type IV collagen.

PMID: 3311751 by Brazel D., et al. Completion of the amino acid sequence of the alpha 1 chain of human basement membrane collagen (type IV) reveals 21 non-triplet interruptions located within the collagenous domain.

54 Publications of Boster COL4A1 Antibodies & ELISA Kits

PubMed ID: PMID:16191423, Protective effects of blocking renin-angiotensin system on the progression of re...
PubMed ID: 10.3892/mmr.2018.9635, Genistein attenuates renal fibrosis in streptozotocin‑induced diabetic rats
PubMed ID: 10.3892/etm.2016.3218, Protective effects of Danggui Buxue Tang on renal function, renal glomerular mes...
PubMed ID: 10.3390/nu8050276, Sesamin Ameliorates High-Fat Diet–Induced Dyslipidemia and Kidney Injury by Re...
PubMed ID: 10.3109/10799893.2016.1155068, Neutral endopeptidase and natriuretic peptide receptors participate in the regul...
PubMed ID: 10.3109/0886022X.2014.934694, Effects of uPA on mesangial matrix changes in the kidney of diabetic rats
PubMed ID: 10.1590/S0100-879X2010007500132, The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a c...
PubMed ID: 10.1538/expanim.56.11, Severe Insulin Resistance and Moderate Glomerulosclerosis in a Minipig Model Ind...
PubMed ID: 10.1093/toxsci/kfm010, Rosiglitazone Prevents Advanced Glycation End Products–Induced Renal Toxicity ...
PubMed ID: 10.1016/S0002-9440(10)63254-3, Advanced Glycation End-Products Induce Connective Tissue Growth Factor-Mediated ...
PubMed ID: 10.1016/j.phymed.2017.09.001, Cardioprotection of Sheng Mai Yin a classic formula on adriamycin induced myocar...
PubMed ID: 10.1016/j.biopha.2020.110321, Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: ...
PubMed ID: 10.1007/s12020-010-9419-0, Effects of aminoguanidine and vitamin C on collagen type IV in diabetic nephropa...
PubMed ID: 10.1007/s11095-013-1209-y, Electrosprayed Microparticles with Loaded pDNA-Calcium Phosphate Nanoparticles t...
PubMed ID: 10.1007/s11033-012-2321-5, Renoprotective effects of berberine and its possible molecular mechanisms in com...
PubMed ID: -, Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Ident...
PubMed ID: -, Yang, Y., Deng, R., Chen, Z., Yao, L., Yang, X., & Xiang, D. (2021). Piperazine ...
PubMed ID: 33581205, Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L...
PubMed ID: 32475419, Zhou S,Ma Y,Zhao D,Mi Y,Zhang C. Transcriptome profiling analysis of underlying ...
PubMed ID: 30431100, Genistein attenuates renal fibrosis in streptozotocin%u2011induced diabetic rats
PubMed ID: 28656209, Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabet...

*Showing only the more recent 20. More publications can be found for each product on its corresponding product page