Product Info Summary
| SKU: | M01411-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC |
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Product info
Product Name
Anti-Collagen IV COL4A1 Monoclonal Antibody
SKU/Catalog Number
M01411-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Collagen IV COL4A1 Monoclonal Antibody catalog # M01411-1. Tested in IF, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen IV COL4A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01411-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
Q32
Isotype
IgG1
Immunogen
Synthesized peptide derived from human Collagen Type IV AA range: 1600-1669
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01411-1 is reactive to COL4A1 in Human, Mouse, Rat
Calculated molecular weight
160.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01411-1 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF 1:50-200
IHC 1:50-300
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunofluorescence analysis of mouse-spleen tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-Collagen IV COL4A1 Monoclonal Antibody (M01411-1)
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