Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3)

Boster Bio Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3) catalog # M01411. Tested in IF, IHC-P, WB applications. This antibody reacts with Human. Cited in 6 publication(s).

Product Info Summary

SKU: M01411
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IF, IHC-P, WB

Product Name

Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3)

See all Collagen IV alpha 1 products

SKU/Catalog Number

M01411

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3) catalog # M01411. Tested in IF, IHC-P, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01411)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

3G3

Isotype

IgG1

Immunogen

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01411 is reactive to COL4A1 in Human

Applications

M01411 is guaranteed for IF, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunofluorescence, 2μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For COL4A1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02462

Gene ID

1282

Gene Name

COL4A1

Full Name

Collagen alpha-1(IV) chain

Weight

160.611kDa

Superfamily

type IV collagen family

Alternative Names

alpha-1 chain; Arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2 COL4A1 BSVD, BSVD1s, PADMAL, RATOR, COL4A1 collagen type IV alpha 1 chain collagen alpha-1(IV) chain|COL4A1 NC1 domain|arresten|collagen IV, alpha-1 polypeptide|collagen of basement membrane, alpha-1 chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on COL4A1, check out the COL4A1 Infographic

COL4A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for COL4A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01411 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats

Advanced Glycation End-Products Induce Connective Tissue Growth Factor-Mediated Renal Fibrosis Predominantly through Transforming Growth Factor ?-Independent Pathway

MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer

Zhu B, Xu T, Yuan J, Guo X, Liu D. Plos One. 2013 Sep 19;8(9):E76282. Doi: 10.1371/Journal.Pone.0076282. Ecollection 2013. Transcriptome Sequencing Reveals Differences Between Primary And Secondary Hair Follicle-Derived Dermal Papilla Cells Of The...

Wen D, Huang X, Zhang M, Zhang L, Chen J, Gu Y, Hao Cm. Plos One. 2013 Dec 3;8(12):E82336. Doi: 10.1371/Journal.Pone.0082336. Ecollection 2013. Resveratrol Attenuates Diabetic Nephropathy Via Modulating Angiogenesis.

Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.

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5 Customer Q&As for Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3)

Question

We are currently using anti-Collagen IV antibody (monoclonal, 3G3) M01411 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

The anti-Collagen IV antibody (monoclonal, 3G3) (M01411) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-31

Question

We bought anti-Collagen IV antibody (monoclonal, 3G3) for IF on placenta in the past. I am using human, and We intend to use the antibody for WB next. My question regards examining placenta as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

I looked at the website and datasheets of our anti-Collagen IV antibody (monoclonal, 3G3) and it seems that M01411 has been tested on human in both IF and WB. Thus M01411 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-20

Question

My team were satisfied with the WB result of your anti-Collagen IV antibody (monoclonal, 3G3). However we have seen positive staining in placenta secreted using this antibody. Is that expected? Could you tell me where is COL4A1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

According to literature, placenta does express COL4A1. Generally COL4A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL4A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 2844531, 3311751, 3691802, 16481288, 16151532

Boster Scientific Support

Answered: 2020-01-14

Question

We have seen staining in human visceral pleura. What should we do? Is anti-Collagen IV antibody (monoclonal, 3G3) supposed to stain visceral pleura positively?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

From literature visceral pleura does express COL4A1. From Uniprot.org, COL4A1 is expressed in visceral pleura, brain, placenta, among other tissues. Regarding which tissues have COL4A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 2844531, 3311751, 3691802, 16481288, 16151532

Boster Scientific Support

Answered: 2019-06-27

Question

We need using your anti-Collagen IV antibody (monoclonal, 3G3) for laminin interactions studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-09-27

Answer

I appreciate your inquiry. This M01411 anti-Collagen IV antibody (monoclonal, 3G3) is validated on human placenta tissue, lung cancer tissue, hela whole cell lysate, a549 whole cell lysate. It is guaranteed to work for IF, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-09-27

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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M01411
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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