Click image to see more details
Product Info Summary
| SKU: | M01411 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3)
SKU/Catalog Number
M01411
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3) catalog # M01411. Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01411)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
3G3
Isotype
Mouse IgG1
Immunogen
E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01411 is reactive to COL4A1 in Human
Observed Molecular Weight
220 kDa
Calculated molecular weight
160.6 kDa
Background of COL4A1
COL4A1, also known as ICH or Collagen alpha-1 (IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01411 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunofluorescence, 2μg/ml
Positive Control
WB: human HEK293T whole cell, human Hela whole cell, human A549 whole cell
IHC: human lung cancer tissue, human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of Collagen IV using anti-Collagen IV antibody (M01411).
Collagen IV was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Collagen IV Antibody (M01411) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
IHC analysis of Collagen IV using anti-Collagen IV antibody (M01411).
Collagen IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Collagen IV Antibody (M01411) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Western blot analysis of Collagen IV using anti-Collagen IV antibody (M01411).
Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293T whole cell lysate,
Lane 2: human Hela whole cell lysate,
Lane 3: human A549 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Collagen IV antigen affinity purified monoclonal antibody (Catalog # M01411) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 220KD. The expected band size for Collagen IV is at 161KD.
Specific Publications For Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3) (M01411)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3)
Question
We are currently using anti-Collagen IV antibody (monoclonal, 3G3) M01411 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The anti-Collagen IV antibody (monoclonal, 3G3) (M01411) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-31
Question
We bought anti-Collagen IV antibody (monoclonal, 3G3) for IF on placenta in the past. I am using human, and We intend to use the antibody for WB next. My question regards examining placenta as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
I looked at the website and datasheets of our anti-Collagen IV antibody (monoclonal, 3G3) and it seems that M01411 has been tested on human in both IF and WB. Thus M01411 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-20
Question
My team were satisfied with the WB result of your anti-Collagen IV antibody (monoclonal, 3G3). However we have seen positive staining in placenta secreted using this antibody. Is that expected? Could you tell me where is COL4A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
According to literature, placenta does express COL4A1. Generally COL4A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL4A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 2844531, 3311751, 3691802, 16481288, 16151532
Boster Scientific Support
Answered: 2020-01-14
Question
We have seen staining in human visceral pleura. What should we do? Is anti-Collagen IV antibody (monoclonal, 3G3) supposed to stain visceral pleura positively?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
From literature visceral pleura does express COL4A1. From Uniprot.org, COL4A1 is expressed in visceral pleura, brain, placenta, among other tissues. Regarding which tissues have COL4A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 2844531, 3311751, 3691802, 16481288, 16151532
Boster Scientific Support
Answered: 2019-06-27
Question
We need using your anti-Collagen IV antibody (monoclonal, 3G3) for laminin interactions studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
I appreciate your inquiry. This M01411 anti-Collagen IV antibody (monoclonal, 3G3) is validated on human placenta tissue, lung cancer tissue, hela whole cell lysate, a549 whole cell lysate. It is guaranteed to work for IF, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-09-27
