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- Table of Contents
Facts about Corticoliberin.
.
Human | |
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Gene Name: | CRH |
Uniprot: | P06850 |
Entrez: | 1392 |
Belongs to: |
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sauvagine/corticotropin-releasing factor/urotensin I family |
CRF; CRH; ProCorticoliberin; Pro-Corticoliberin
Mass (kDA):
21.422 kDA
Human | |
---|---|
Location: | 8q13.1 |
Sequence: | 8; NC_000008.11 (66176376..66178464, complement) |
Produced by the hypothalamus and placenta.
Secreted.
The CRH indicator is a highly sensitive protein that detects corticosterone. Researchers are increasingly using CRH markers to identify this particular protein. The antibody is made with high-affinity primary antibodies and is available to order in multiple sizes. It has many clinical applications, including detection of corticosterone plasma and serum. Researchers can submit results to be published in scientific journals. This service is open to all scientists worldwide.
This rabbit polyclonal antibody recognizes HumanCRH protein. This antibody has been tested by immunoassay, immunofluorescence and Western Blot. It can also be used for immunohistochemistry. A blocking peptide, which can recognize a specific length of immunogen, can be purchased to validate further. It can be kept at -20°C for one year.
This antiCorticoliberin anti-Corticoliberin anti-CRH antibody was created by pre-proCRH Expression in Escherichiacoli. The chimeric protein was injected into rabbits and used as an immunogen. Immunoassays and immunocytochemical techniques were used to characterize the antibodies against the neuropeptide moiety. These antibodies showed cross-reactivity with the full-length CRH precursor as well as its cleavage product. They did not react with antagonist CRH(1-41).
Boster Bio is an antibody manufacturer that specializes in the production of picogram-sensitive ELISA kits and research antibodies. The company's extensive catalogue includes over 12,000 antibodies. It has been validated for WB, IHC, and Flow. Boster Bio antibodies are based on insights from immunogen design, and support provided by Sanbio, a BeNeLux distributor. You can be sure that a high-quality antibody will deliver results when you require it.
The hypothalamus secretes CRH, a 41-amino acid peptide. As its levels have decreased, this hormone is thought to be associated with Alzheimer’s disease. An autosomal-recessive disorder of hypothalamus can lead to multiple metabolic consequences including hepatitis or hypoglycemia.
High-affinity primary antibodies against the CRH markers can be used for a variety diagnosis applications, including the detection CRH-positive cancer cell lines. This marker can be found on microglial brain cells and is associated in poor prognosis. The CRH molecule can bind to both CRHR1 as well as CRHR2, with the former having a greater affinity than the latter. Cancer cells that express CRHR1 are associated to poor prognosis. Furthermore, Minas et al. showed that CRH increased Fas expression in ovarian carcinoma cells through CRHR1, and it potentiates apoptosis in peripheral blood lymphocytes.
The CRH-1f could be detected in the majority the TL samples. Seven of nine TL sample samples contained the CRH–R1g and the CRH–R1c. The CRH-1e and CRH-1g were not detected in two-thirds (33%) of the samples. Therefore, the CRH–R1b (and CRHR–R1f) are more likely not to be detected in tissues from both TL- and non-pregnant mouse.
High-affinity primary antibodies for the CRR2 receptor have been shown to detect CRR2 within human myometrium. Its use in pregnancy research stems from the findings of researchers who used it to monitor human fetal development. The CRH-2 receptor is a key component for the placental time clock. It determines how long a woman has to gestate and when she should give birth.
Affinity refers the strength to which a single molecule interacts with a ligand. The equilibrium dissociation coefficient (KD), which measures the rate at ligand and molecule binding, is used to determine affinity. Because affinity binding can be reversed, the concentrations of reactants affect the rate at which the antibodies bind the ligand. The rate at which these molecules are dissociated is the same as their rates of occurrence. A smaller KD value means higher affinity.
The affinity of a primary antibody to a ligand binds to it. The lower the KD value, it is more ligand-affine. KD and affinity show an inverted relationship. As such, antibodies that bind a ligand with a higher affinity than those that bind a protein with a lower affinity. The UC Davis scientists used the exact same techniques to measure an antibody's affinity to the CRH ligand.
The IgD class BCR is required to affinity mature insulin-IgM. InsAKLH was injected on days 0, 21 & 42 into hematopoietic tissue of mice lacking IgD. Serum immunoglobulin titers were determined by ELISA using a biotin-binding substrate.
The CRH marker, a blood marker that is used to diagnose different types of depression, is used. It has been shown highly to correlate with SST/VIP/PVALB. In addition to these markers, CRH expression is also related to gene expression of inflammation-related genes, including NF-kB. This study examined the cellular as well as laminar specificity for CRH expression in MDD-related patients.
MDD patients are more likely to express CRH in their sgACC than healthy controls. The expression of CRH was not significantly different from the healthy control group. MDD patients have a decreased expression of cellular CRH in the pyramidal neurons and GABAergic interneuron. This study demonstrates the importance of CRH for MDD diagnosis.
The present study has many limitations. The sample size is relatively small and may have caused type II errors. All patients were hospitalized, which may have affected the DEX/CRH test's sensitivity. The sample may be biased towards those with severe depression, especially in patients with chronic depression. The study also included patients who were taking antidepressants. This may have affected the sensitivity of CRH markers.
The CRH biomarker is a great state-dependent marker. The DEX/CRH is useful for monitoring abnormalities in the HPA-axis in patients suffering from major depression. It can also be used to determine if a patient is taking medication, age, and gender. It is important to note that DEX/CRH tests are sensitive and state-dependent. Clinical applications of CRH markers are largely limited to diagnosing mood disorders or psychiatric symptoms.
While the FDA has not approved use of the CRH marker to detect early cancers, there are some targeted panel options available for colorectal or bladder cancer. For detection of cirrhosis and hepatocellular, the CE-IVD-marked blood tests for Septin 9methylation are also available. Liquid biopsy is another promising technique. John Hopkins University has created a series liquid biopsy products, including UroSEEK PapSEEK & CancerSEEK.
Although the CRH marker is a good indicator of CRH in women and men, its effects on patients aged over 60 years and younger are still not fully understood. The study also shows the possible role of the hormone CRH in gender-gender variations in hormonal responses. Studies on CRH concentrations showed that females have higher levels. However, the statistical difference between the two hormones wasn't significant.
PMID: 6605851 by Shibahara S., et al. Isolation and sequence analysis of the human corticotropin-releasing factor precursor gene.
PMID: 2783917 by Robinson B.G., et al. Preprocorticotropin releasing hormone: cDNA sequence and in vitro processing.