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Product Info Summary
| SKU: | A00629-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Corticoliberin CRH Antibody
SKU/Catalog Number
A00629-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Corticoliberin CRH Antibody (Catalog # A00629-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
CRH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Corticoliberin CRH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00629-2)
Host
Rabbit
Contents
CRH antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH. The immunogen is located within amino acids 30 - 80 of CRH.
Cross-reactivity
CRH antibody is human specific.
Reactive Species
A00629-2 is reactive to CRH in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
21.4 kDa
Background of CRH
Corticotropin-releasing hormone (CRH) is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress (1). In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta where it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturitio (2). Marked reduction in this protein has been observed in association with Alzheimer disease (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00629-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CRH in human placenta tissue lysate with CRH antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of CRH in human brain tissue with CRH antibody at 5 μg/mL.
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Immunofluorescence of CRH in human brain tissue with CRH antibody at 20 μg/mL.
Specific Publications For Anti-Corticoliberin CRH Antibody (A00629-2)
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