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- Table of Contents
Facts about Cone-rod homeobox protein.
Essential for the maintenance of mammalian photoreceptors. .
Human | |
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Gene Name: | CRX |
Uniprot: | O43186 |
Entrez: | 1406 |
Belongs to: |
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paired homeobox family |
cone-rod homeobox; CORD2; CRDcone-rod homeobox protein; CRX; LCA7; LCA7orthodenticle homeobox 3; OTX3
Mass (kDA):
32.261 kDA
Human | |
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Location: | 19q13.33 |
Sequence: | 19; NC_000019.10 (47821937..47843324) |
Retina.
Nucleus.
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The CRX Marker can be used to detect specific kinds of cancer. The staining of CRX by immunohistochemistry revealed that expression was evident in tumor nuclei but not in optic nerve cells. This suggests that CRX could be used to target therapeutic intervention in pineal paraenchymal tumors. The staining of CRX can be used to differentiate between benign tumors and malignant tumors.
The CRX gene is responsible for the production of the cone-rod homeobox protein. The protein is found in the retina, the light-sensitive tissue at the back of the eye. The protein binds with specific regions of DNA and regulates the activity of certain genes. This protein is referred to as a transcription factor. In studies, it is used to analyze the function of genes. The CRX gene is found in almost every cell of the human body.
CRX has a transactivating activity and binds to photoreceptor-specific gene promoters. It interacts with another gene that is the neural retina leucine zipper proteins (NRL), which is a transcription factor comparable to AP-1. These proteins collaborate to activate the rhodopsin promotion. However, the transactivating function is not fully understood.
Many tumors can be associated with CRX-mediated expression. This marker is especially useful in diagnosing pineal tumors where intranuclear immunereactivity is absent. Therefore, the CRX marker should be considered an appropriate routine diagnostic marker. While non-TF markers aren't yet widely utilized, they haven't reached the level of acceptance that would allow for routine use. It is therefore crucial to be aware of the best uses for the CRX Marker.
We previously identified two chromatin-bound protein, Nrl, and Crx by combing the antibodies. These antibodies are highly affine for CRX-binding proteins , but are not deterministic. Therefore, it is essential to understand how CRX-mediated chromatin enrichment differs from CRX-binding nonspecific proteins.
The CRX-marker allows researchers to distinguish between these two types of primary antibodies the CRX marker can also differentiate between them. Primary antibodies are the most effective reagents for bioanalysis, since they recognize a unique epitope that has high specificity and affinity. Secondary antibodies can be utilized in conjunction with primary antibodies to increase the signal and the CRX marker is an excellent tool for bioinformatics research.
Monoclonal antibodies recognize CRX as photoreceptor gene. This gene regulates the expression of several genes. It is crucial for photoreceptor differentiation as mutant mice are unable to form the outer segments, which are the organelles that are sensitive to light. They are also unable to determine the function of the photoreceptor. CRX monoclonal antibodies are useful in identifying retinas that have CRX mutations in mice.
The affinity of monoclonal antibodies for the peptide is proportional its concentration. Therefore, KD values directly relate to the amount of antigen. The antibody's affinity is higher when the KD value is lower. The measurement and data processing was carried out at UC Davis. Abcam scientists examined the results and came to their conclusions. A KD below 10 milliM strongly suggests a greater affinity for antibodies.
The CRX binding site is extremely conserved. Its binding preference isn't consistent with bicoid's, another homeodomain transcription factor. Bicoid and CRX share a regularity of helical periodicity. In addition, CBRs are tolerant of the wide range of spacings and orientations for their binding sites. CBRs were tested for binding sites that have an affinity higher than 0.05 of the consensus binding site. The position of the nucleotide was used to count the number of binding sites bound by CRX.
Additionally, Crx and Nr2e3 can interact in vitro. In vitro co-immunoprecipitation assays have verified these interactions. Mutations of these proteins did not demonstrate an interaction, however the presence of Nr2e3 and Crx in a cultured cell model suggests that the two molecules interact in vitro. Additionally, the antibodies recognized mutant Nr2e3 proteins in bovine retinal nuclear extracts.
CRX Marker is a molecular-biology technique used in many applications. Scientists can alter DNA and other genetic material to study various diseases and understand how they impact our bodies. Boster provides extensive technical resources for scientists, including blogs and disease information, along with web-based digital tools. These include high-quality buffers designed for lysis that enable researchers to get high-quality results and minimize cross-linking intensity.
These tips can be helpful when you are having issues with your CRX marking device. First, you must close the program. It could be affected if the program status is "Not Responding"/"Running". Try closing the program and re-starting the computer. If this doesn't resolve the issue, then continue to investigate the CRX marker.
PMID: 9390563 by Freund C.L., et al. Cone-rod dystrophy due to mutations in a novel photoreceptor-specific homeobox gene (CRX) essential for maintenance of the photoreceptor.
PMID: 17286855 by Roni V., et al. Mapping of transcription start sites of human retina expressed genes.