Product Info Summary
| SKU: | M02202 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CRX Monoclonal Antibody
SKU/Catalog Number
M02202
BM5783 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CRX Monoclonal Antibody catalog # M02202. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRX Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02202)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFIO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CRX Binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, e.g. NRL and RX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors.
Reactive Species
M02202 is reactive to CRX in Human
Observed Molecular Weight
37 kDa
Calculated molecular weight
32.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02202 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: Y79 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CRX expression in Y79 cell lysate.
Specific Publications For Anti-CRX Monoclonal Antibody (M02202)
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Customer Q&As
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4 Customer Q&As for Anti-CRX Monoclonal Antibody
Question
Would M02202 anti-CRX Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
As indicated on the product datasheet, M02202 anti-CRX Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-23
Question
Is a blocking peptide available for product anti-CRX Monoclonal antibody (M02202)?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We do provide the blocking peptide for product anti-CRX Monoclonal antibody (M02202). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-06
Question
I would like to test anti-CRX Monoclonal antibody M02202 on human retina for research purposes, then I may be interested in using anti-CRX Monoclonal antibody M02202 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The products we sell, including anti-CRX Monoclonal antibody M02202, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-29
Question
See attached the WB image, lot number and protocol we used for retina using anti-CRX Monoclonal antibody M02202. Please let me know if you require anything else.
E. Wu
Verified customer
Asked: 2014-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-28


