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- Table of Contents
1 Citations 7 Q&As
Facts about C-C motif chemokine 27.
Binds to CCR10. .
Human | |
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Gene Name: | CCL27 |
Uniprot: | Q9Y4X3 |
Entrez: | 10850 |
Belongs to: |
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intercrine beta (chemokine CC) family |
ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27
Mass (kDA):
12.618 kDA
Human | |
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Location: | 9p13.3 |
Sequence: | 9; NC_000009.12 (34661890..34662657, complement) |
Testis, thymus, placenta, ovary and skin.
Secreted.
Boster Bio CCL27 offers an antibody for a wide array of applications. These include cancer diagnostics and research in rheumatoid-arthritis. CCL27 is a blood protein that regulates immune function. This antibody can also be found in neutralizing antibodies (anti-CCL5 or metabolites). Boster scientists can buy these antibodies around the globe.
CCL27, a protein that reacts against Human CTACK, is known as CCL27. CCL27 receptors are expressed in the skin, and in cancer cells. It plays many roles in immunity and tumor progression. Additionally, it regulates proliferation as well as metastasis. This protein can be used in cancer diagnosis by determining the CCL27 level and homing into the inflammatory environment. Its predictive ability is still unknown.
The DNA sequence encoding CCL27 chemokine protein was isolated from genomic or cDNA libraries. DNA encoding CCL27 was amplified using standard amplification techniques. The sequences were amplified by PCR. The CCL27 marker in Boster Bio could be used as an easy monitoring biomarker during routine examinations. This marker can also be used in conjunction with existing biomarkers. These biomarkers are complex and expensive, so they can be difficult to identify.
CCR10 is a receptor that CCL27 and CCR10 share, but the functions of these receptors are not known. Both are important for skin immunity as well as inflammation. The CCR10/CCL27 Axis is believed to regulate migration of skin T cells. Their role in the development skin disease is not yet known. It is important to understand how these two proteins interact in vivo in order to determine which treatments will be most effective.
A binding agent is the CCL28 chemokine-protein complex. It allows for the measurement of CCL28 mixed with other substances. The binding agent can be either an antibody or a fragment thereof, such as a Fab fraction. It should be a monoclonal antibody to cross-reactivity testing. The CCL27 complex of chemokine proteins contains amino acids that are closely related CCL28.
The CCL27 marker is a chemokine produced by the keratinocytes in the skin. It is known to attract T-cells to the skin, which can play a role in inflammation. CCL27 is strongly associated with disease activity and pruritus. These clinicopathologic characteristics of this marker include:
High-affinity antibodies against CCL27 are possible only if the antigen being targeted is chosen carefully. The antigen might be expressed in fusion or tagged with an Epitope. The antibody will recognize it and neutralize it. The antibody will then bind to the second binding ligand, which can be detected visually or by instrument means. There are many methods and formats for measuring CCL27-chemokine proteins.
Purified antibodies derived from the CCL27 chemokine and CCL28 can be used for affinity chromatography. They also may be useful in binding research. The antibodies can also be used as diluents for pharmaceutical compositions. High-affinity primary antibodies based upon this marker are not yet commercially available. They are however available in different purity levels.
Antibodies that target the CCL27 chemokine marker have another advantage: they can detect subpopulations. These probes can distinguish cell types and tissues. They are not extremely specific. They are therefore excellent for studying CCL27's role in viral replication. To find high-affinity antibodies against the CCL27 chemokine marker, you can use primers that complement the two 5' regions of the DNA region.
Another advantage of antibodies that are anti-CCL27 chemokine marker, is the possibility to screen expression banks with different antigens. Antibodies to CCL28 are typically labeled in order to allow detection of antigens by antibody binding. In addition, antibodies against CCL27 chemokine marker can be useful in diagnosing disease. Antibodies raised against these chemical chemokines can also be used to detect diseases due to their overproduction.
You can immobilize a CCL27 chemokine on many matrices. Plastic, beads, filters, gels and beads are all suitable matrices. Different methods can be used to separate the immobilized ligand/receptor combinations. Biotin-avidin-ligands are used to create high-affinity antibody. The CCL27 chemokine is a common chemokine that is expressed in a variety of cell types.
CCL27 markers are a small multifunctional proteins that play multiple roles in human well-being. It regulates immune responses and plays an important role for T cell development, maturation. Trafficking and function. This chemokine has been implicated in the progression and metastasis of cancers, tumor-induced apoptosis, and regulation of proliferation and metastasis. CCL27 is present in many tissues, such as skin, and binds directly to the CCR10 chemokine receptor. However, it's not clear how predictive value it has.
The CCL27 marker was found to increase T cell migration. TCR-10 cells are responsible for the chemoattractive properties of CCL27. They are found in high numbers in the skin. Hence, clinical applications of the CCL27 marker are likely to be quite diverse. It is unknown how CCL27 affects cancer cells. However, data suggests that CCL27 may be associated with tumor-associated death.
In addition to its potential role in the detection of cancer, CCL27 is also an excellent biomarker for NPC. It was found to be significantly greater in EBV infected and VCA IgA+ individuals. CCR10 was also found to be responsible for increased production of CCL27. This protein is upregulated in immortalized T cells. NPC patients had plasma CCL27 levels that were significantly higher than those of healthy donors and controls.
The plasma concentration of CCL27 protein was determined using double-antibody sandwich ELISA. This allowed for statistical analysis. The CCL27 (mouse anti-human CCL27) was used as the capture antibody. It was 4.0 mg/ml. The test samples were then added overnight to 96-well microplates. After two hours, cells were incubated overnight with the detection antibody, a biotinylated goat-anti-human CCL27 at 75 mg/ml.
In a recent study, neonatal mice exposed primary keratinocytes to IL-1b (20 ng/ml). Supernatants showed elevated levels of CCL27 protein. Similar results were found in another experiment, where skin extracts were incubated with the same concentrations of TNF-a and IL-1b. This suggests that CCL27 could play a role at the initiation and maintenance of inflammation.
PMID: 10556532 by Ishikawa-Mochizuki I., et al. Molecular cloning of a novel CC chemokine, interleukin-11 receptor alpha-locus chemokine (ILC), which is located on chromosome 9p13 and a potential homologue of a CC chemokine encoded by molluscum contagiosum virus.
PMID: 10588729 by Morales J., et al. CTACK, a skin-associated chemokine that preferentially attracts skin- homing memory T cells.
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