Human CCL27/CTACK ELISA Kit PicoKine™

Human CCL27/CTACK ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0688
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human CCL27/CTACK ELISA Kit PicoKine™

See all CCL27/CTACK products

SKU/Catalog Number

EK0688

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CCL27/CTACK ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CCL27/CTACK ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0688)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F25-G112

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0688 is reactive to CCL27 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0688 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0688-CAP Anti-Human CCL27 Pre-coated 96-well strip microplate 1
EK0688-ST Human CCL27 Standard 2 vials, 10 ng/tube
EK0688-DA Human CCL27 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)02000200020002000200020002000
O.D.0.0260.0440.0730.1510.3910.7421.5702.418

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CCL27/CTACK ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8624084685239777
Standard deviation4.9810.5660.065.7813.8662.93
CV (%)5.8%4.4%7.1%6.8%5.8%8.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL27/CTACK in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 186989592924.434.8%
Sample 22402492562452475.852.3%
Sample 384685295589088543.394.9%
*number of samples for each test n=16.

Gene/Protein Information For CCL27 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y4X3

Gene ID

10850

Gene Name

CCL27

Full Name

C-C motif chemokine 27

Weight

12.618kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27 CCL27 ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27 C-C motif chemokine ligand 27 C-C motif chemokine 27|CC chemokine ILC|IL-11 R-alpha-locus chemokine|IL-11 Ralpha-locus chemokine|chemokine (C-C motif) ligand 27|cutaneous T-cell attracting chemokine|skinkine|small inducible cytokine subfamily A (Cys-Cys), member 27|small-inducible cytokine A27

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL27, check out the CCL27 Infographic

CCL27 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human CCL27/CTACK ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

T. Liu

Verified customer

Asked: 2020-02-10

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-02-10

Question

Q: how do I analyze ELISA data? I measured CCL27 level in plasma.

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-06-05

Question

Q: Can CCL27 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-05-13

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CCL27 using the EK0688 Human CCL27 Picokine® ELISA Kit?

P. Young

Verified customer

Asked: 2018-05-15

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-05-15

Question

Q: What is the optimal O.D. value for CCL27 ELISA kit? I performed your CCL27 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL27 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-03-15

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL27 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CCL27 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-03-15

Question

Q: can I use citrate plasma as samples in Human CCL27 Picokine® ELISA Kit (Catalog # EK0688)?

Verified Customer

Verified customer

Asked: 2018-03-11

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CCL27 causing degradation of its protein structure. CCL27 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CCL27 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-03-11

Question

Q: if the enzyme conjugated CCL27 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL27 antigen?

C. Campbell

Verified customer

Asked: 2016-07-26

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-07-26

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