Mouse CCL27/CTACK ELISA Kit PicoKine™

Mouse CCL27/CTACK ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

Product Info Summary

SKU: EK0689
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CCL27/CTACK ELISA Kit PicoKine™

See all CCL27/CTACK products

SKU/Catalog Number

EK0689

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CCL27/CTACK ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CCL27/CTACK ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0689)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L26-N120

Sensitivity

<12 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0689 is reactive to Ccl27 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0689 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0689-CAP Anti-Mouse Ccl27 Pre-coated 96-well strip microplate 1
EK0689-ST Mouse Ccl27 Standard 2 vials, 10 ng/tube
EK0689-DA Mouse Ccl27 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04000200040002000400020004000
O.D.0.0270.1000.1390.2260.4200.7751.3001.906

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CCL27/CTACK, Dilution ratio of 1:1, concentration in serum is around 2ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CCL27/CTACK ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)18041417131883931657
Standard deviation9.1824.0199.3511.8429.47107.7
CV (%)6.5%%5.8%5.8%7.5%%7.5%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL27/CTACK in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11801782001891868.694.6%
Sample 241443840237540722.685.5%
Sample 31713170816661742170727.121.5%
*number of samples for each test n=16.

Gene/Protein Information For Ccl27 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Z1X0

Gene ID

Gene Name

Ccl27

Full Name

C-C motif chemokine 27

Weight

13.464kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl27, check out the Ccl27 Infographic

Ccl27 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Mouse CCL27/CTACK ELISA Kit PicoKine™

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-06-18

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-01-07

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-01-07

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-04-07

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-04-07

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CCL27 using the EK0689 Mouse CCL27 Picokine® ELISA Kit?

S. Mitchell

Verified customer

Asked: 2018-01-24

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-01-24

Question

Q: how can I thaw whole blood sample for CCL27 ELISA after freezing?

L. Gonzalez

Verified customer

Asked: 2016-12-18

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL27 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-12-18

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

K. Gonzalez

Verified customer

Asked: 2016-11-23

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-11-23

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