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We validate the specificity of these antibodies to DDB2 by testing them on tissues known to express DDB2 positively and negatively. Browse below to find the DDB2 antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.
If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.
Facts about DNA damage-binding protein 2.
Needed for DNA repair.Binds to DDB1 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex).
The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches.
|WD repeat DDB2/WDR76 family|
damage-specific DNA binding protein 2 (48kD); damage-specific DNA binding protein 2, 48kDa; Damage-specific DNA-binding protein 2; DDB p48 subunit; DDB2; DDBB; DNA damage-binding protein 2; FLJ34321; UV-damaged DNA-binding protein 2; UV-DDB 2; UV-DDB2; xeroderma pigmentosum group E protein; XP5; XPE/DDB2
|Sequence:||11; NC_000011.10 (47214942..47239218)|
Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.
Nucleus. Accumulates at sites of DNA damage following UV irradiation.
The DDB2 marker is used to detect anti-DNA damaging proteins. The boster bio DDB2 antigen was tested in IF and WB applications and reacts with Human, Mouse, and Rat. In addition this antibody is offered in a range of dilutions, allowing researchers to test a large range of biological samples.
This Antibody recognizes the DNA damage-binding protein 2 (DDB2) it is an integral component of heterodimeric DNA repair proteins. Boster Bio's Anti DNA damage-binding protein2 DDB2 Marker reacts to human samples, mouse and rat. This antibody is compatible with different cell lines which makes it suitable to be used in a variety of applications.
DDB2 is a major player in Nucleotide Excision Repair (NER) which is a crucial DNA repair pathway that is activated following damage to DNA. DDB2 enhances the efficiency of NER and DNA synthesis through the regulation of the p21Waf1/Cip1 protein, which helps ensure an efficient repair process. In addition, DDB2 reduces p21Waf1 through proteolysis and aids in cell death in the event of DNA damage.
Anti-DNA damage-binding protein 2 (DDB2) has been shown to increase global genome repair and shield cells from UV radiation. The overexpression of mutant DDB2 in HeLa cells prevents death caused by UV. DDB2 that is mutant is not capable of DDB1-DDB1 interaction or damage recognition, but it does protect cells against UV radiation.
ASO blocks the expression of DDB2 and reduces the protective effect. The overexpression of DDB2 in hamster cells could not be able to stop UV-induced death. Although it may play a role in UV protection, DDB2 overexpression in cFLIP-lacking cell cultures did not cause the accumulation of cFLIP.
Boster Bio's Anti-DNA Damage binds protein 2 (DDB2) has high sensitivity in many cell types. DDB2 is crucial to UV resistance. However, it was unable to revert activated apoptosis in Drosophila. It is important to remember that DDB2 functions differently in different cell types. The cell make-up of different species could contribute to the DDB2-mediated response to UV stress.
The DDB2 overexpression inhibits the cleavage and degradation of PARP and DFF under UV. DDB2 overexpression in HeLa cells enhances UV resistance. This was correlated with cFLIP. The protective effect was observed within 36 hours after infection. DDB2 overexpression was linked to an increase in the rate of apoptosis of HR18 cells.
The anti-DDB2 antibodies produced by Boster Bio is used in a variety of applications. Its high affinity primary antibodies have been validated in ELISA, Western Blotting, and Immunohistochemistry. The vast array of antibodies it has covers nearly every gene in the mouse and human genomes. The boster bio DDB2 antibody has been approved to be used in all research applications, including immunohistochemistry and Western Blotting.
The DDB2 marker is an indicator of DNA binding. It has many applications in neuroscience, biology, and cancer. Boster Bio developed a variety of products that measure the presence of proteins in samples, including ELISA kits, primary antibodies, and biochemical Reagents. These instruments are extremely sensitive and can detect biomarkers using picogram-level sensitiveness. Boster Bio is a leading provider of anti-DNA damage-binding proteins antibodies as well as ELISA kits.