Product Info Summary
SKU: | A01430-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-DDB2 Antibody Picoband™
SKU/Catalog Number
A01430-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDB2 Antibody Picoband™ catalog # A01430-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDB2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01430-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DDB2 recombinant protein (Position: Q10-R424).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01430-2 is reactive to DDB2 in Human
Applications
A01430-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
48 kDa
Calculated molecular weight
47.864kDa
Background of XPE/DDB2
DNA damage-binding protein 2 is a protein that in humans is encoded by the DDB2 gene. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. And this subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DDB2 using anti-DDB2 antibody (A01430-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U2OS whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB2 antigen affinity purified polyclonal antibody (Catalog # A01430-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB2 at approximately 48KD. The expected band size for DDB2 is at 48KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of HeLa cells using anti-DDB2 antibody (A01430-2).
Overlay histogram showing HeLa cells stained with A01430-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDB2 Antibody (A01430-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For DDB2 (Source: Uniprot.org, NCBI)
Gene Name
DDB2
Full Name
DNA damage-binding protein 2
Weight
47.864kDa
Superfamily
WD repeat DDB2/WDR76 family
Alternative Names
damage-specific DNA binding protein 2 (48kD); damage-specific DNA binding protein 2, 48kDa; Damage-specific DNA-binding protein 2; DDB p48 subunit; DDB2; DDBB; DNA damage-binding protein 2; FLJ34321; UV-damaged DNA-binding protein 2; UV-DDB 2; UV-DDB2; xeroderma pigmentosum group E protein; XP5; XPE/DDB2 DDB2 DDBB, UV-DDB2, XPE damage specific DNA binding protein 2 DNA damage-binding protein 2|DDB p48 subunit|UV-DDB 2|UV-damaged DNA-binding protein 2|damage-specific DNA binding protein 2, 48kDa|xeroderma pigmentosum group E protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDB2, check out the DDB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDB2 Antibody Picoband™ (A01430-2)
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