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- Table of Contents
Facts about Microprocessor complex subunit DGCR8.
Within the microprocessor complex, DGCR8 be the molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a combined trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active.
Human | |
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Gene Name: | DGCR8 |
Uniprot: | Q8WYQ5 |
Entrez: | 54487 |
Belongs to: |
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No superfamily |
C22orf12; DGCRK6chromosome 22 open reading frame 12; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; microprocessor complex subunit DGCR8; pasha
Mass (kDA):
86.045 kDA
Human | |
---|---|
Location: | 22q11.21 |
Sequence: | 22; NC_000022.11 (20080232..20111877) |
Ubiquitously expressed.
Nucleus. Nucleus, nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies (PubMed:17159994).
You may have heard of the DGCR8 marker, and are looking to get a sample. This article will provide basic information about the protein and its uses. It will also provide information about the Boster bio product line. We'll go over some of the most well-known uses for the DGCR8 mark. Below are some of the most commonly used applications for this protein.
Scientists have recently discovered the function of DGCR8 in embryonic stem cell differentiation. The DGCR8 gene is part of the SUMO1 family and assists the RNase III enzyme Drosha to process microRNAs. The DGCR8 marker, also referred to as DGCR, is essential for the biogenesis of microRNAs, which are short non-coding RNAs. The DGCR8 gene, which is expressed in ES cells and neural stem cells, is vital for embryonic stem cell differentiation. Boster Bio developed a knockout model of the DGCR8 gene. It was found that DGCR8 is necessary for the biogenesis and expression of miRNAs. DGCR8 knockout mice lack the DGCR8 gene, but they express differentiation markers. Dicer1 knockout cells are also available.
The DGCR8 antibody is highly specific in its anti-human glycophorin A (or CD235a) marker. The Boster Bio antibody reacts with human cells in a non-hazardous way. It is formulated in PBS 10mM containing 0.05 percent BSA and azide. The antibody is validated for use in immunohistochemistry, Western Blotting and ELISA.
Boster Bio Anti–GAPDH Antibody Picoband is available under catalog number A00227-1. It reacts with Human, Mouse, & Rat and is a monoclonal, non-immunoglobulin, anti-mouse antibody. The antibody is made up of 5mg BSA, and is compatible with mouse cells and rats. It binds to the DGCR8 gene in the spinal cord and increases the function of cells mediated by ERK.
Whether you are using this antibody to analyze the behavior of a cell, test a new treatment for cancer, or perform basic research to discover more about a specific gene, Boster Bio has a solution. The company offers high-affinity primary antibodies that have been validated on Western Blotting, Immunohistochemistry, and ELISA. The antibodies have been tested in more than 4,000 research papers and are widely used by the research community.
PMID: 12705904 by Shiohama A., et al. Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region.
PMID: 15589161 by Landthaler M., et al. The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis.