Anti-DGCR8 Antibody Picoband™

DGCR8 antibody

Boster Bio Anti-DGCR8 Antibody Picoband™ catalog # A00475-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00475-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC, ICC, WB

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Product Name

Anti-DGCR8 Antibody Picoband™

View all DGCR8 Antibodies

SKU/Catalog Number

A00475-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DGCR8 Antibody Picoband™ catalog # A00475-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DGCR8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00475-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human DGCR8 recombinant protein (Position: K561-Q762).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00475-1 is reactive to DGCR8 in Human, Mouse, Rat

Applications

A00475-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

86.045kDa

Background of DGCR8

The DGCR8 microprocessor complex subunit (DiGeorge syndrome chromosomal [or critical] region 8) is a protein that in humans is encoded by the DGCR8 gene. This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For DGCR8 (Source: Uniprot.org, NCBI)

Gene Name

DGCR8

Full Name

Microprocessor complex subunit DGCR8

Weight

86.045kDa

Alternative Names

C22orf12; DGCRK6chromosome 22 open reading frame 12; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; microprocessor complex subunit DGCR8; pasha DGCR8 C22orf12, DGCRK6, Gy1, pasha DGCR8 microprocessor complex subunit microprocessor complex subunit DGCR8|DiGeorge syndrome critical region 8|DiGeorge syndrome critical region gene 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DGCR8, check out the DGCR8 Infographic

DGCR8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DGCR8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-DGCR8 Antibody Picoband™

Question

We are currently using anti-DGCR8 antibody A00475-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-01-31

Answer

The anti-DGCR8 antibody (A00475-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-31

Order DetailsPrice
A00475-1

100μg

$370
A00475-1-10ug

10μg sample (liquid)

$99
A00475-1-Biotin

100 μg Biotin conjugated

$570
A00475-1-Cy3

100 μg Cy3 conjugated

$570
A00475-1-Dylight488

100 μg Dylight488 conjugated

$570
A00475-1-Dylight550

100 μg Dylight550 conjugated

$570
A00475-1-Dylight594

100 μg Dylight594 conjugated

$570
A00475-1-FITC

100 μg FITC conjugated

$570
A00475-1-HRP

100 μg HRP conjugated

$570
A00475-1-APC

100 μg APC conjugated

$670
A00475-1-PE

100 μg PE conjugated

$670
A00475-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A00475-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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