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Product Info Summary
| SKU: | M00475 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-DGCR8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00475
BM4373 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DGCR8 Rabbit Monoclonal Antibody catalog # M00475. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DGCR8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00475)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EAO-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DGCR8
Reactive Species
M00475 is reactive to DGCR8 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
86.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00475 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of DGCR8 expression in (1) HEK293 cell lysate; (2) HeLa cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Specific Publications For Anti-DGCR8 Rabbit Monoclonal Antibody (M00475)
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4 Customer Q&As for Anti-DGCR8 Rabbit Monoclonal Antibody
Question
Does M00475 anti-DGCR8 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
It shows on the product datasheet, M00475 anti-DGCR8 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-27
Question
Do you have a BSA free version of anti-DGCR8 Rabbit Monoclonal antibody M00475 available?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DGCR8 Rabbit Monoclonal antibody M00475 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-05
Question
Can you help my question with product M00475, anti-DGCR8 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00475 anti-DGCR8 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-01
Question
Does anti-DGCR8 Rabbit Monoclonal antibody M00475 work for WB with salivary gland?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
According to the expression profile of salivary gland, DGCR8 is highly expressed in salivary gland. So, it is likely that anti-DGCR8 Rabbit Monoclonal antibody M00475 will work for WB with salivary gland.
Boster Scientific Support
Answered: 2018-05-17
