Anti-DGCR8 Rabbit Monoclonal Antibody

DGCR8 antibody

Boster Bio Anti-DGCR8 Rabbit Monoclonal Antibody catalog # M00475. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00475
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, ICC, WB

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Product Name

Anti-DGCR8 Rabbit Monoclonal Antibody

View all DGCR8 Antibodies

SKU/Catalog Number

M00475

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DGCR8 Rabbit Monoclonal Antibody catalog # M00475. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DGCR8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00475)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EAO-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DGCR8

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00475 is reactive to DGCR8 in Human, Mouse, Rat

Applications

M00475 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

501 kDa

Calculated molecular weight

86.045kDa

Background of DGCR8

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:100-1:500
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For DGCR8 (Source: Uniprot.org, NCBI)

Gene Name

DGCR8

Full Name

Microprocessor complex subunit DGCR8

Weight

86.045kDa

Alternative Names

C22orf12; DGCRK6chromosome 22 open reading frame 12; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; microprocessor complex subunit DGCR8; pasha DGCR8 C22orf12, DGCRK6, Gy1, pasha DGCR8 microprocessor complex subunit microprocessor complex subunit DGCR8|DiGeorge syndrome critical region 8|DiGeorge syndrome critical region gene 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DGCR8, check out the DGCR8 Infographic

DGCR8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DGCR8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-DGCR8 Rabbit Monoclonal Antibody

Question

Does M00475 anti-DGCR8 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

It shows on the product datasheet, M00475 anti-DGCR8 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-27

Question

Do you have a BSA free version of anti-DGCR8 Rabbit Monoclonal antibody M00475 available?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DGCR8 Rabbit Monoclonal antibody M00475 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-05

Question

Can you help my question with product M00475, anti-DGCR8 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00475 anti-DGCR8 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-01

Question

Does anti-DGCR8 Rabbit Monoclonal antibody M00475 work for WB with salivary gland?

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

According to the expression profile of salivary gland, DGCR8 is highly expressed in salivary gland. So, it is likely that anti-DGCR8 Rabbit Monoclonal antibody M00475 will work for WB with salivary gland.

Boster Scientific Support

Answered: 2018-05-17

Order DetailsPrice
M00475

100μL

$370
M00475-30ul

30μL sample

$99
M00475-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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Eddy test
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M00475
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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