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DUSP6 / Dual specificity protein phosphatase 6 · IHC design guide
Design Immunohistochemistry for DUSP6
Plan paraffin-section DUSP6 IHC around the cytoplasmic staining reported across most tissues (HPA tissue IHC). Compare glandular-cell staining in colon with adipocytes, where staining was not detected, and keep fixation consistent across samples (HPA tissue IHC; standard IHC practice).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
DUSP6 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasm in most tissues (HPA tissue IHC) | |
| Staining pattern | Cytoplasmic staining across most tissues (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet A02157-2) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | Adipose tissue+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across samples. (standard IHC practice; not target-specific) | |
| Caveat | Staining and RNA expression have medium consistency (HPA tissue IHC) | |
| Regulation | Expression regulation is unreported (UniProt) | |
| Isoform / epitope | 2 isoforms; no extracellular domain; epitopes unmapped (UniProt) |
Section 1
Recommended DUSP6 IHC & IF Protocols
The catalog antibody’s IHC-P protocol (datasheet A02157-2) is accompanied by published DUSP6 IHC methods for heart sections (PMC8631968) and clinical tissue sections (PMC12420332).
| Sample | Paraffin-embedded human liver cancer tissue; fixative not specified (datasheet A02157-2) |
| Fixation | Image fixative and duration unreported (datasheet A02157-2); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet A02157-2); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet A02157-2) |
| Primary antibody | Rabbit anti-DUSP6, 2-5 μg/ml (datasheet A02157-2) |
| Primary incubation | Overnight at 4 °C (datasheet A02157-2) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet A02157-2) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | DUSP6-positive staining in glandular cells of adrenal gland (HPA tissue IHC: Medium). HPA tissue profile: Cytoplasmic expression in most tissues. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA retrieval at pH 8.0 (datasheet A02157-2); use the published citrate retrieval conditions when reproducing either article (PMC8631968; PMC12420332).
Section 2
What Is the Expected DUSP6 Staining Pattern?
DUSP6 is a cytoplasmic protein with no transmembrane segment (UniProt Q16828: subcellular location and topology). In paraffin sections, expect cytoplasmic staining in many tissues, including glandular cells, respiratory epithelial cells, hematopoietic cells, and glial cells represented in the HPA tissue panel (HPA: tissue IHC). Treat this as a pattern guide rather than a guarantee for every section: HPA rates its tissue IHC evidence Approved, with medium consistency between staining and RNA expression (HPA: tissue IHC).
| Discrete cytoplasmic signal in glandular cells of adrenal gland, appendix, breast, colon, or duodenum (HPA: Medium). | Consistent with the reported DUSP6 tissue pattern (HPA: cytoplasmic expression in most tissues). Judge the stained cells and compartment together; signal anywhere in the tissue alone is insufficient for a positive call (general IHC practice). |
| Predominantly nuclear or membranous staining, without a convincing cytoplasmic component. | Recheck specificity and image interpretation: UniProt places DUSP6 in the cytoplasm and reports no transmembrane segment (UniProt Q16828). Nuclear staining alone is inconclusive, because HPA reports uncertain nucleoplasmic localization in ICC-IF (HPA: subcellular). |
| Strong signal confined to adipocytes or esophageal squamous epithelial cells (HPA: Not detected). | This disagrees with those HPA cell-level observations; assess cross-reactivity, endogenous detection activity, and tissue identification (HPA: tissue IHC; general IHC practice). HPA's ratings describe its sampled material, not every specimen. |
| Diffuse chromogen across cells, stroma, or the entire section, with little cell-level distinction. | A broad haze cannot establish DUSP6 localization (general IHC practice). Check background controls before scoring it; nonspecific binding or detection-system background can obscure the cytoplasmic pattern (general IHC practice). |
| No signal in a suitable reference section containing adrenal gland glandular cells or bronchial respiratory epithelium (HPA: Medium). | Review tissue preservation, antibody conditions, detection, and controls before calling the specimen negative (general IHC practice). HPA reports Medium staining in those cell types, but its Approved rating has only medium RNA agreement (HPA: tissue IHC). |
💡Expected DUSP6 appearanceCall a result positive when identifiable cells show predominantly cytoplasmic chromogenic staining, such as Medium signal in glandular cells of the HPA-listed tissues; isolated nuclear, membranous, or diffuse background signal requires further checks (HPA: tissue IHC; UniProt Q16828: cytoplasm; general IHC practice).
| Reference tissue and cell selection (HPA: tissue IHC) | HPA reports Medium staining in adrenal and appendix glandular cells, bronchial respiratory epithelium, bone marrow hematopoietic cells, and cerebral cortex glial cells. It reports Not detected in adipocytes and esophageal squamous epithelial cells; compare the specified cells rather than whole-tissue darkness (HPA: tissue IHC). |
| Evidence strength and antibody choice (HPA: tissue IHC and antibodies) | HPA labels the tissue pattern Approved, with medium consistency against RNA data; CAB017566 is listed as IHC Approved (HPA: antibodies). These labels support cautious pattern comparison, not an assumed result for another antibody or every sample (HPA: tissue IHC). |
| Cellular localization and protein topology (UniProt Q16828; HPA: tissue IHC) | UniProt assigns DUSP6 to the cytoplasm and reports no transmembrane segment; HPA describes cytoplasmic expression in most tissues (UniProt Q16828; HPA: tissue IHC). Neither source supports treating a sharp membrane rim as the expected IHC pattern. |
| Isoforms and processing (UniProt Q16828) | UniProt lists two isoforms, one chain spanning residues 1–381, and no signal peptide or propeptide (UniProt Q16828). The payload gives no epitope location, so it cannot predict whether an antibody detects both isoforms or whether retrieval favors either one. |
| IF/ICC Q&A: should nuclear fluorescence count as the expected result? (HPA: subcellular) | HPA supports cytosolic ICC-IF localization and marks nucleoplasmic localization uncertain; two listed antibodies have Enhanced ICC validation (HPA: subcellular; HPA: antibodies). Interpret any nuclear signal with that caveat. IF/ICC assay setup belongs in its separate guide. |
| Situation | Likely cause | Next action |
|---|---|---|
| Reference positive tissue shows no cytoplasmic stain (HPA: Medium in selected cells). | A failed stain, unsuitable reference section, or an antibody mismatch is possible (general IHC practice). | Verify the cell type, a working positive control, antibody identity, and the IHC-validated antibody's stated conditions; then review retrieval and detection settings (general IHC practice). No DUSP6-specific retrieval requirement is supplied. |
| Signal appears only in nuclei or along cell borders. | That distribution conflicts with cytoplasmic IHC expectations; nucleoplasmic ICC-IF localization remains uncertain (UniProt Q16828; HPA: tissue IHC and subcellular). | Compare cell morphology and controls, then review antibody specificity and detection background (general IHC practice). Do not use uncertain ICC-IF nuclear localization to validate a nuclear-only IHC result. |
| Adipocytes or esophageal squamous epithelial cells stain strongly (HPA: Not detected). | Cross-reactivity or endogenous detection activity may produce unexpected chromogen (general IHC practice). | Run an appropriate primary-antibody omission control and review blocking and detection steps (general IHC practice). Compare the same cell types with HPA observations before assigning DUSP6 positivity. |
| The section has uniform haze that hides cell boundaries. | Nonspecific binding, incomplete blocking, or detection background can reduce contrast (general IHC practice). | Inspect omission and background controls; adjust blocking, antibody concentration, washing, and chromogen development within the assay's validated range (general IHC practice). Score only identifiable cell-associated signal. |
| A weak signal is seen in a tissue expected to stain at Medium level (HPA: tissue IHC). | Cell composition or technical variation may differ from HPA's sampled sections (HPA: tissue IHC; general IHC practice). | Confirm that the reported positive cell population is present; compare a reference section stained in the same run and review counterstain and detection settings (general IHC practice). |
| An IF/ICC image seems inconsistent with the paraffin-section IHC pattern. | HPA records supported cytosol and uncertain nucleoplasm for ICC-IF, while its tissue IHC summary is cytoplasmic (HPA: subcellular; HPA: tissue IHC). | Interpret each assay against its own HPA evidence and controls (HPA: tissue IHC and subcellular; general IHC practice). Consult the separate IF/ICC guide for that assay; do not infer an IF/ICC protocol from this IHC section. |
Experiment evidence
IHC & IF images showing DUSP6 staining
A02157-2 · IHCIHC analysis of DUSP6 using anti-DUSP6 antibody (A02157-2). DUSP6 was detected in a paraffin-embedded section of human liver cancer tissue.
A02157-2 · IHCIHC analysis of DUSP6 using anti-DUSP6 antibody (A02157-2). DUSP6 was detected in a paraffin-embedded section of human liver cancer tissue.
A02157-2 · IFIF analysis of DUSP6 using anti-DUSP6 antibody (A02157-2). DUSP6 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen
M02157 · IHCImmunohistochemical analysis of paraffin-embedded human thyroid, using DUSP6 Antibody.
M02157 · IFImmunofluorescent analysis using the Antibody at 1:150 dilution.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000139318-DUSP6/tissue/cerebral+cortex#imid_4574416
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000139318-DUSP6/tissue/colon#imid_4574334
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000139318-DUSP6/tissue/liver#imid_4574327
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000139318-DUSP6/tissue/kidney#imid_4574328
HPA · ICC-IF CACO-2Human Protein Atlas immunofluorescence, CACO-2, antibody HPA053188; green = DUSP6, blue = nucleus, red = microtubules.
HPA · ICC-IF CACO-2Human Protein Atlas immunofluorescence, CACO-2, antibody HPA053188; green = DUSP6, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for DUSP6 IHC & IF
🧪Run adrenal gland first and assess its glandular cells for DUSP6 staining (HPA: Medium in adrenal gland glandular cells); use adipose tissue adipocytes as the negative tissue (HPA: Not detected in adipocytes). On the adrenal slide, cells without specific staining should show only background signal, but the supplied HPA rows do not identify a particular adrenal cell type as an internal biological negative (HPA: adrenal gland glandular cells only).
Positive control tissue: Adrenal gland (Glandular cells, HPA Medium)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show DUSP6 in CACO-2, PC-3, U2OS, SK-MEL-30, with annotated localisation: Nucleoplasm (uncertain), Cytosol (supported) (HPA subcellular).
Technical controls: Include no-primary (secondary-only) and host- and clonality-matched rabbit IgG controls, alongside DUSP6-knockout tissue or a validated peptide-block control (standard IHC practice; selected-SKU caption: rabbit anti-DUSP6 antibody). For adrenal gland, check endogenous peroxidase and biotin background before interpreting SABC/DAB staining (standard IHC practice; selected-SKU caption: SABC/DAB detection).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected-SKU paraffin-section caption does not report a fixative (selected-SKU caption: fixative not stated). The caption uses heat retrieval in pH 8.0 EDTA, but whether DUSP6 detection depends on that retrieval condition is unreported (selected-SKU caption: EDTA heat retrieval). The supplied evidence does not establish whether frozen sections or IF are easier; assess adrenal background from endogenous peroxidase or biotin when using the caption’s SABC/DAB method (standard IHC practice; selected-SKU caption: SABC/DAB detection).
HPA tissue IHC evidence for DUSP6
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Approved — Medium consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | Medium | Protein (IHC) | HPA → |
| Appendix | Glandular cells | Medium | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Breast | Glandular cells | Medium | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Cervix | Squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Esophagus | Squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Ovary | Ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Vagina | Squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced DUSP6 IHC Tips
Troubleshoot DUSP6 staining in paraffin sections by checking retrieval, cellular localisation, controls, and scoring before interpreting differences between samples.
What retrieval should I try first when DUSP6 staining is weak in paraffin sections?
Start with heat-mediated retrieval in EDTA buffer at pH 8.0 (datasheet A02157-2). The selected paraffin-section image used this retrieval before overnight incubation with 2 μg/ml antibody at 4°C (caption A02157-2). If staining remains weak, compare retrieval duration on adjacent sections while keeping antibody concentration, detection, and development constant (standard IHC practice). Include a tissue compartment expected to stain, such as glandular cells in colon, to distinguish poor retrieval from a low-expression sample (HPA: Medium in colon glandular cells). Excessive heat can damage morphology, so inspect both staining and tissue integrity after each adjustment (standard IHC practice).
Could fixation explain weak or uneven DUSP6 staining?
The selected paraffin-section caption does not state a fixative, so target-specific DUSP6 fixation sensitivity is unknown (caption A02157-2). Record the fixative and fixation duration for each specimen, then compare sections processed with the same retrieval and antibody conditions (standard IHC practice). Uneven fixation can produce regional staining differences, but those differences alone do not establish a DUSP6-specific fixation effect (standard IHC practice). Check preserved morphology and a concurrently processed positive control before increasing antibody concentration or DAB development time (standard IHC practice). Keep the selected pH 8.0 EDTA retrieval as the starting condition during that comparison (datasheet A02157-2).
Where should convincing DUSP6 staining appear in chromogenic IHC?
Expect predominantly cytoplasmic staining: DUSP6 is annotated as cytoplasmic, and HPA describes cytoplasmic expression in most tissues (UniProt Q16828; HPA: tissue IHC profile). HPA also reports supported cytosolic and uncertain nucleoplasmic localisation in its subcellular data, so isolated nuclear DAB staining needs cautious interpretation (HPA: subcellular). DUSP6 has no transmembrane segment or signal peptide; a sharp membrane-only pattern therefore warrants a specificity check (UniProt Q16828 topology and processing). Compare stained cells with local tissue morphology and a matched negative control, particularly when pigment or precipitate resembles intracellular DAB (standard IHC practice). Score cytoplasmic and nuclear signals separately if both persist after control review (standard IHC practice).
Can isoforms or epitope accessibility account for discordant DUSP6 IHC results?
DUSP6 has 2 annotated isoforms, but the supplied antibody caption does not identify its epitope or establish isoform-specific recognition (UniProt Q16828 isoforms; caption A02157-2). The protein contains a rhodanese domain at residues 30–148 and a phosphatase domain at 206–349; those positions alone cannot identify what the antibody detects (UniProt Q16828 domains). Check the catalog epitope information before interpreting discordant staining as an isoform difference (standard IHC practice). Compare adjacent sections using the documented pH 8.0 retrieval and consistent detection to assess technical variation (datasheet A02157-2; standard IHC practice). If available, use an independently validated antibody with a known, distinct epitope to investigate persistent disagreement (standard IHC practice).
How should I investigate DUSP6 staining with multiplex IF?
Use IF as a separate validation experiment alongside chromogenic IHC, whose selected paraffin-section example uses DAB detection (caption A02157-2). Pair DUSP6 with an epithelial marker when examining glandular cells, and evaluate whether the signals occur in the same cells rather than assuming every cell is positive (HPA: Medium in colon glandular cells; standard IF practice). Choose spectrally separated fluorophores and consider a far-red DUSP6 channel when tissue autofluorescence affects shorter wavelengths (standard IF practice). Permeabilise sufficiently for intracellular access because supported DUSP6 localisation is cytosolic; optimise the treatment if nuclear signal is also assessed (HPA: subcellular; standard IF practice). Include single-label and secondary-only controls to identify bleed-through and nonspecific fluorescence (standard IF practice).
How can I reduce diffuse DAB background without losing DUSP6 signal?
The selected paraffin-section procedure blocked with 10% goat serum and applied 2 μg/ml primary antibody overnight at 4°C (caption A02157-2). Begin troubleshooting by checking the no-primary control, wash steps, and DAB development time while preserving the documented retrieval condition (standard IHC practice; datasheet A02157-2). Block endogenous peroxidase before chromogenic detection and assess endogenous biotin background if using the caption's streptavidin–biotin detection system (caption A02157-2; standard IHC practice). Titrate primary antibody downward if background remains broad, comparing adjacent sections under identical development conditions (standard IHC practice). A credible signal should retain cellular structure and predominantly cytoplasmic distribution (HPA: tissue IHC profile).
How should I score DUSP6 IHC across specimens with different cellular composition? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and compartment before scoring because HPA describes cytoplasmic expression in most tissues but varying detection across cell types (HPA: tissue IHC profile). Record the percentage of positive target cells and staining intensity, or calculate a cytoplasmic H-score from intensity classes 0–3 across 0–100% of target cells (standard IHC scoring practice). Normalise counts to the number of evaluable target cells, or report positive-cell density per mm² of viable tissue when spatial abundance matters (standard IHC scoring practice). Exclude necrotic and folded regions using the same criteria in every specimen (standard IHC practice). Keep retrieval, antibody concentration, imaging, and scoring thresholds consistent across the comparison (standard IHC practice).
When is an apparent DUSP6-positive focus more likely to be artefact?
Treat predominantly cytoplasmic signal in intact cells as more consistent with the reported IHC pattern than membrane-only staining or isolated nuclear precipitate (HPA: tissue IHC profile; UniProt Q16828 topology). Check whether positive cells fit the tissue context: HPA reports medium staining in colon glandular cells and no detection in adipocytes (HPA: tissue IHC). Signal restricted to section edges, folds, or necrotic areas warrants review of morphology and controls before biological interpretation (standard IHC practice). Compare no-primary and peroxidase-blocked controls when DAB appears in unexpected cells, since endogenous enzyme activity can mimic chromogenic positivity (standard IHC practice). HPA rates tissue staining as Approved with medium consistency against RNA expression, so interpret a single discordant focus cautiously (HPA: tissue IHC reliability).
Boster reagents
Best DUSP6 / Dual specificity protein phosphatase 6 IHC Antibodies
Catalog antibodies A02157-2 and M02157 have paraffin-section IHC images from human tissue (IHC image captions); A02157-2 also has an IF/ICC image from PC-3 cells (IF image caption).
Real IHC data
Anti-DUSP6 Antibody ®
Cat # A02157-2
Real IHC data
Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody
Cat # M02157
A02157-2 has IHC images from paraffin-embedded human liver cancer, colonic adenocarcinoma, and rectal cancer tissue, plus an IF/ICC image from PC-3 cells (A02157-2 image captions). M02157 has an IHC image from paraffin-embedded human thyroid and is listed for IF/ICC, though its IF captions identify no sample (M02157 image captions; catalog applications).
Which to pick: For tissue IHC, choose A02157-2 when its documented paraffin-section retrieval and detection conditions are useful; M02157 offers a rabbit monoclonal option with a paraffin-embedded human thyroid image (A02157-2 IHC image captions; M02157 catalog and IHC caption). For IF/ICC, A02157-2 has the more informative image caption because it identifies PC-3 cells and the staining conditions (A02157-2 IF image caption; M02157 IF image captions). Both list human, mouse, and rat reactivity, but the supplied IHC images show human tissue only; the fixative is unreported for both (catalog reactivity; IHC image captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q16828 (DUS6_HUMAN, Dual specificity protein phosphatase 6).
- Human Protein Atlas. DUSP6 tissue IHC expression (reliability: Approved).
- Human Protein Atlas. DUSP6 subcellular location (ICC-IF): Localized to the nucleoplasm and cytosol..
- Human Protein Atlas. DUSP6 antibody validation summary (3 antibodies).
- Sleeve Gastrectomy Ameliorates Diabetes-Induced Cardiac Hypertrophy Correlates With the MAPK Signaling Pathway. Frontiers in physiology 2021 — PMC8631968.
- Inhibition of DUSP6 sensitizes ovarian cancer cells to chemotherapeutic agents via regulation of ERK signaling response genes. Oncotarget 2019 — PMC6534361.
- A novel prognostic biomarker DUSP6 promote the malignant progression of bladder cancer through mTOR mediated mitophagy. Frontiers in oncology 2025 — PMC12420332.
- Upregulation of cGMP-dependent Protein Kinase (PRKG1) in the Development of Adolescent Idiopathic Scoliosis. Orthopaedic surgery 2020 — PMC7454216.
- PubMed PMID:8670865 — UniProt-cited evidence.
- PubMed PMID:9858808 — UniProt-cited evidence.
- PubMed PMID:15489334 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for DUSP6 (Dual specificity protein phosphatase 6). Expected localisation Cytoplasm in most tissues (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet A02157-2). Validated IHC antibody: A02157-2 at 2-5 μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative Adipose tissue. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human liver cancer tissue; fixative not specified (datasheet A02157-2); 2. Fixation: Image fixative and duration unreported (datasheet A02157-2); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet A02157-2); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet A02157-2); 8. Primary antibody: Rabbit anti-DUSP6, 2-5 μg/ml (datasheet A02157-2); 9. Primary incubation: Overnight at 4 °C (datasheet A02157-2). Watch for: Fixation: Keep fixation consistent across samples. (standard IHC practice; not target-specific); Caveat: Staining and RNA expression have medium consistency (HPA tissue IHC); Isoform / epitope: 2 isoforms; no extracellular domain; epitopes unmapped (UniProt); Regulation: Expression regulation is unreported (UniProt). Evidence: UniProt Q16828, HPA tissue IHC · Approved, HPA ICC-IF · nucleoplasm and cytosol., Datasheet A02157-2, PMC8631968, PMC12420332. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/DUSP6/immunohistochemistry-ihc-guide.