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Product Info Summary
| SKU: | M02157 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02157
BM4283 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody catalog # M02157. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02157)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DBE-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DUSP6
Reactive Species
M02157 is reactive to DUSP6 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02157 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: NIH/3T3 cell
IHC: human thyroid tissue
Validation Images & Assay Conditions
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Western blot analysis of DUSP6 expression in NIH/3T3 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded human thyroid, using DUSP6 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody (M02157)
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Customer Q&As
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3 Customer Q&As for Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-DUSP6/Mkp 3 Rabbit Monoclonal antibody for IHC for rat kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat kidney identification?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
It shows on the product datasheet, M02157 anti-DUSP6/Mkp 3 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-DUSP6/Mkp 3 Rabbit Monoclonal antibody M02157 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
J. Zhao
Verified customer
Asked: 2018-04-05
Answer
The anti-DUSP6/Mkp 3 Rabbit Monoclonal antibody (M02157) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-05
Question
I have a question about product M02157, anti-DUSP6/Mkp 3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Krishna
Verified customer
Asked: 2014-03-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02157 anti-DUSP6/Mkp 3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-03-14
