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- Table of Contents
Facts about Dentin matrix acidic phosphoprotein 1.
During the osteoblast to osteocyte transition phase it is phosphorylated and exported to the extracellular matrix, where it regulates nucleation of hydroxyapatite. .
Human | |
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Gene Name: | DMP1 |
Uniprot: | Q13316 |
Entrez: | 1758 |
Belongs to: |
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No superfamily |
ARHP; ARHR; dentin matrix acidic phosphoprotein 1; dentin matrix acidic phosphoprotein; Dentin matrix protein 1; DMP1; DMP-1
Mass (kDA):
55.782 kDA
Human | |
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Location: | 4q22.1 |
Sequence: | 4; NC_000004.12 (87650280..87664357) |
Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.
Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
If you're looking for a boster antibodies to the DMP1 proteins, you've come the right place. The boster protein capture technique uses anti-DDK affinity columns and conventional chromatography steps to make the process easy and convenient for all scientists around the world. Continue reading to learn more about this unique method. It is applicable to all types of scientists, including genometypers and histologists.
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Immunohistochemistry, which uses antibodies to stain cells, is a unique method. This technique detects antibodies and haptens in cells. Boster Bio uses FFPE cells pellets and optimises antibody dilutions for each customer. The company uses a high throughput screening process to make antibodies and will also accept smear slides from customers. Boster Bio's Immunohistochemistry service is supported by technical assistance provided by Sanbio (a distributor of BeNeLux)
Histology is a branch in biology that is crucial to the study of cells. To understand a disease in detail, it is necessary to study how cells develop. It also helps to know how the organs of the body develop over time. For example, the cells in the brain are a complex network of tissues called a neuromuscular junction, which is an important part of the immune system.
Alleles of the human antigen p53 are found in the DMP1 gene. Alleles bind together to the same sequence under hybridization conditions. This makes genetic associations between patients with genes much easier. However, the DMP1 gene is not universally conserved. It is still widely used in many types of research, despite this. Below are the top uses of the DMP1 genealogist marker.
To detect the DMP1 protein, hDPSCs were cultured on glass slides and fixed with methanol at -20 degC for 10 min. They were then rinsed in phosphate buffered saline containing Tween-20. The cells were then incubated in 1:200, 1:50, and 1:50 dilutions respectively of primary antibodies. Both DSPP and DMP1 antibodies were used to detect DMP-1. Secondary antibodies were used with dilutions at 1:500.
After incubating the samples with primary antibodies, they were then incubated with secondary monoclonal antibodies (rabbit polyclonal Npt2a/b) and anti-mouse IgG/Ets1 monclonal antibody DABPA, respectively. After this, they were exposed to X-ray film to visualize the signals. Finally, densitometric quantification was performed using ImageJ software from the National Institutes of Health.
DMP1-pcDNA3.1 constructs revealed that DMP1 is primarily located in the nuclei and cytoplasm. In MC3T3E1 cells the NH2-terminal part was detected. Transfected cells containing DMP1 pcDNA3.1 showed greater amounts of green and yellow cytoplasm. It is possible that DMP1pcDNA3.1 contains processed fragments which are not detected with DMP1pcDNA3.1 antibodies.
DMP1 plays an important role in cell cycle regulation. DMP1 gene has a high number of copies on human chromosome 7, which is believed to act as a tumor suppressor. The protein binds both non-americ consensus DNA sequencing and GGA cores. It also interacts well with Ets family transcript factors. DMP1 does interact with cyclin dependent kinases.
ARF-mediated Apoptosis refers to a mechanism that involves activating a gene known as ARF. ARF-induced cell loss is a result if the survival factors in cell culture medium partially suppress the apoptotic signals. DMP1-mediated Apoptotic pathway results in cell death.
The DMP1 gene encodes large peptides that are present in bone matrix in four forms. These include a 57 kDa Cterminal fragment, 37 KDa N-terminal core proteins, and glycosylated DMP1PG. DMP1-PG is highly expressed in osteoid cartilage, and the Ser-Gly-Ala sequence is conserved across species.
DMP1 is abundant in bone. However, N-terminal fragments of its protein are only found on unmineralized bone surface surfaces. These fragments were first observed in newly formed osteoid. They were also found in the matrix and articular cartilage. Although the exact function of DMP1 proteins is still unknown, it is a promising indicator of bone growth.
The S89G-DMP1 mutation has been confirmed by genotyping. The S89G–Dmp1 mice produced the acidic proteins. The S89G–Dmp1 mice produced the same acids. They were then compared to WT controls. The DMP1 protein could then be detected by Stains-All staining. Figure 1C illustrates the expression profiles for DMP1 in both genotypes.
To monitor osteoid growth, the DMP1 gene is used. DMP1-PG would be absent in bone, which would result in osteoid formation. This gene is also vital for osteoinduction and biomineralization. DMP1 is a biomarker that helps bone formation in many clinical settings. And, it is a highly versatile protein with a plethora of applications.
PMID: 9177774 by Hirst K.L., et al. Elucidation of the sequence and the genomic organization of the human dentin matrix acidic phosphoprotein 1 (DMP1) gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II.
PMID: 8586437 by Aplin H.M., et al. Mapping of the human dentin matrix acidic phosphoprotein gene (DMP1) to the dentinogenesis imperfecta type II critical region at chromosome 4q21.