Mouse DMP-1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse DMP-1. 96wells/kit, with removable strips.
Mouse DMP-1 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma(heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse DMP-1 ELISA Kit PicoKine™
See all DMP-1 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse DMP1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. DMP1 tissue specificity: Expressed in tooth particularly in odontoblast, ameloblast and cementoblast. Also expressed in bone particularly in osteoblast.|
|Cite This Product||Mouse DMP-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1290)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: L17-Y503|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Dmp1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||13406|
|Species Of This Entry||Mouse|
|Protein Name||Dentin matrix acidic phosphoprotein 1|
|Alternative Names||DMP-1|ARHP; ARHR; dentin matrix acidic phosphoprotein 1; dentin matrix acidic phosphoprotein; Dentin matrix protein 1; DMP1; DMP-1|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Methylation; Reduction; Phosphorothioation; Dephosphorylation; Ubiquitination|
|Gene Location||5 E5|5 50.61 cM, on Chromasome 5, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Dmp1 (Source: Uniprot.org, NCBI)
|Protein Function||May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity). .|
|Tissue Specificity||Expressed in tooth particularly in odontoblast, ameloblast and cementoblast. Also expressed in bone particularly in osteoblast.|
|Related Diseases||Rickets; Hypophosphatemic Rickets; Familial Hypophosphatemic Bone Disease; Hypophosphatemia; Neoplasms; Malignant Neoplasms; Osteomalacia; Autosomal Recessive Hypophosphatemic Vitamin D Refractory Rickets; Dentinogenesis Imperfecta; Hypertrophy; Kidney Diseases; Cell Transformation, Neoplastic; Tissue Adhesions; Dentin, Secondary; Osteoporosis; Fracture; Chronic Kidney Disease; Carcinoma; Osteopenia|
|Related Pathways||Dentinogenesis; Odontoblast Differentiation; Regeneration; Osteoblast Differentiation; Bone Mineralization; Dentin Mineralization; Localization; Cell Proliferation; Secretion; Cell Differentiation; Ossification; Cell Cycle; Bone Remodeling; Bone Resorption; Cell Growth; Pathogenesis; Excretion; Bone Development; Odontogenesis; Cell Cycle Arrest|
|Background||Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein(SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.|
|Scientific References||PMID: 9525343 by McDougall M., et al. Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues.|
PMID: 17033621 by Feng J.Q., et al. Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism.
Kit Components And Assay QC Data Details Of Mouse DMP-1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1290-CAP||96-well plate precoated with anti-Mouse DMP-1 antibody||1|
|EK1290-ST||lyophilized recombinant Mouse DMP-1 standard||10ng/tube|
|EK1290-DA||biotinylated anti-Mouse DMP-1 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1290 Mouse DMP-1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse DMP-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse DMP-1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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