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- Table of Contents
Designing a Western blot for Alpha-enolase? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ENO1 Western Blot Guide1 Citations
Facts about Alpha-enolase.
Stimulates immunoglobulin production. .
| Human | |
|---|---|
| Gene Name: | ENO1 |
| Uniprot: | P06733 |
| Entrez: | 2023 |

| Belongs to: |
|---|
| enolase family |

2-phospho-D-glycerate hydro-lyase; Alpha enolase; C-myc promoter-binding protein; c-myc promoter-binding protein-1; EC 4.2.1; EC 4.2.1.11; ENO1L1; Enolase 1; enolase 1, (alpha); MBP-1; MBPB1; MPB-1; MPB1c-myc promoter-binding protein 1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; PPHalpha enolase like 1; tau-crystallin
Mass (kDA):
47.169 kDA

| Human | |
|---|---|
| Location: | 1p36.23 |
| Sequence: | 1; NC_000001.11 (8861000..8878686, complement) |
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.; [Isoform MBP-1]: Nucleus.






PMID: 3529090 by Giallongo A., et al. Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase.
PMID: 2373081 by Giallongo A., et al. Structure of the human gene for alpha-enolase.
*More publications can be found for each product on its corresponding product page