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Product Info Summary
| SKU: | M01250 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody
SKU/Catalog Number
M01250
BM4004 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody catalog # M01250. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01250)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEA-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ENO1
Reactive Species
M01250 is reactive to ENO1 in Human, Mouse, Rat
Observed Molecular Weight
47 kDa
Calculated molecular weight
47.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01250 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human MCF-7 whole cell, human Hela whole cell, human U251 whole cell, human A549 whole cell, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of ENO1 using anti-ENO1 antibody (M01250).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1 antigen affinity purified monoclonal antibody (M01250) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using ENO1 Antibody.
Specific Publications For Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody (M01250)
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Customer Q&As
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7 Customer Q&As for Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody
Question
Will M01250 anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
As indicated on the product datasheet, M01250 anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-16
Question
My question regarding product M01250, anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01250 anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-24
Question
Is this M01250 anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody reactive to the isotypes of ENO1?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The immunogen of M01250 anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody is A synthesized peptide derived from human ENO1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-08
Question
Does anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody M01250 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
According to the expression profile of lung, ENO1 is highly expressed in lung. So, it is likely that anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody M01250 will work for WB with lung.
Boster Scientific Support
Answered: 2019-08-19
Question
Is there a BSA free version of anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody M01250 available?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody M01250 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-27
Question
I was wanting to use your anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
R. Li
Verified customer
Asked: 2018-02-23
Answer
You can see on the product datasheet, M01250 anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-ENO1/Alpha Enolase Rabbit Monoclonal antibody M01250. Let me know if you need anything else.
K. Kulkarni
Verified customer
Asked: 2016-10-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-10
