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Product Info Summary
| SKU: | PROTP06733 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Enolase 1/Non-neural enolase Recombinant Protein
View all ENO1 recombinant proteins
SKU/Catalog Number
PROTP06733
Size
Starting from 20ug
Tag
Non-Tagged
Description
Human Enolase 1/Non-neural enolase Recombinant Protein expressed in E. coli (non-tagged). Sequence domain: 1-434aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human Enolase 1/Non-neural enolase Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP06733)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
47.2 kDa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Human, P06733, 1-434aa; MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAKBioactivity
Specific activity is > 20,000 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For Human Enolase 1/Non-neural enolase Recombinant Protein (PROTP06733)
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