FADD Antibodies

FAS-associated death domain protein (FADD)

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 into the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation.

Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a part in the positive regulation of interferon signaling.

Gene Information

Human
Gene Name:	FADD
Uniprot:	Q13158
Entrez:	8772

Family

Belongs to:
No superfamily

Alternative Names

FADD; Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; GIG3; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; mediator of receptor-induced toxicity; MGC8528; MORT1; MORT1Fas-associating protein with death domain; protein FADD

Molecular Weight

Mass (kDA):
23.279 kDA

Genomic Context

Human
Location:	11q13.3
Sequence:	11; NC_000011.10 (70203296..70207390)

Tissue Specificity

Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Leukemia
• Malignant Paraganglionic Neoplasm
• Inflammation
• Lymphoma
• Mammary Neoplasms
• Malignant Neoplasm Of Prostate
• Prostatic Neoplasms
• Adenocarcinoma
• Malignant Neoplasm Of Breast
• Liver Carcinoma

• Malignant Squamous Cell Neoplasm
• Autoimmune Reaction
• Cell Transformation, Neoplastic
• Neoplasm Metastasis
• Malignant Tumor Of Colon
• Malignant Neoplasm Of Lung
• Glioma

Interacting Proteins

• CASP8
• FAS
• Fas
• FASLG
• LRRK2

• MBD4
• MYD88
• PARK7
• PLK1
• RIPK1

• SDCBP
• TRADD

Pathways

• Cell Death
• Induction Of Apoptosis
• Cell Cycle
• Cell Proliferation
• Pathogenesis
• Programmed Cell Death
• Localization
• Sensitization
• Cell Growth
• Immune Response
• Autophagy
• Apoptotic Process
• Cell Killing

• Release Of Cytochrome C From Mitochondria
• Secretion
• Rna Interference
• Transport
• Necroptosis
• Proteolysis
• Cell Activation

PTMs

• Cleavage
• Phosphorylation
• Ubiquitination
• Methylation
• Oxidation
• Dephosphorylation
• Acetylation
• Biotinylation
• Demethylation

• Nitrosylation
• Reduction
• Phosphorothioation
• Acylation
• Ribosylation
• Glycosylation
• Deacetylation
• Palmitoylation
• Myristoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FADD

Best Uses of the FADD Marker

The FADD Marker is among the most frequently used proteins in cell biology, but how can you determine if it's appropriate for your research? There are several methods to do this, including apoptosis, Western Blotting, Immunohistochemistry, and ELISA. This article will review some of these methods. Additionally, you'll be taught about Boster Bio-engineering, apoptosis and mAbs.

Boster Bio-engineering

One of the primary sources of characteristics for workers is the database O*NET. This database lists the main characteristics of workers such as gender, age, education and gender. Boster Bio-engineering has created the FADD Marker to gauge these characteristics. There is a drawback. In some cases, the FADD Marker may be inaccurate. In these situations, the Boster bio-engineering FADD Marker could cause bias in the results.

DD domain

Boster offers high-affinity primary antibodies that are widely employed in research. Their antibodies are supported by a high level of scientific credibility. They have been validated in Western blotting, immunohistochemistry, and ELISA protocols. Boster also offers FADD Marker kits, reagents, and kits. We will review the best uses of FADD-Marker in the research community.

Apoptosis

FAS-associated mortality domain protein (23 kDa) is an adaptor protein that plays an important role in extrinsic and intrinsic Apoptosis. It also plays a role in necroptosis. It binds to Fas receptors and causes apoptosis by causing trimerization of the DD domain. The initiator triggers caspases 8 and 10 to cut off the DD domain.

Cell lysates were made using RIPA buffer, which contained tablets that inhibit protease. After centrifugation of the samples, they were subjected SDS-PAGE using a denaturing 12% SDS Polyacrylamide gel. After that, the cells were blocked using Tris-buffered saline , containing 0.05 percent Tween-20 and five% non-fat milk. Antibody detection was done using the BCA assay kit, and a NEPER nuclear cytoplasm fraction kit.

mAbs

The most common therapeutic use of the FADD marker is for diagnosing cancer. The mAbs are capable of being able to recognize a variety of molecules. These molecules have a wide spectrum of applications, including the identification of disease markers and hormones, allergens and vitamins. Drug discovery and development are also therapeutic uses for the FADD marker. They are also commonly used in the pharmaceutical field as carriers or vehicles for other drugs. They are among the fastest growing areas of the biological pharmaceutical industry.

In addition to the use of mAbs to determine FADD, these markers can also be used to assess the importance of other proteins in cancer. FADD has been shown to be linked to CD95 as well as an apoptosis-associated proteins and other Apoptotic proteins. However, there is a significant amount of FADD that is not redirected to DISC. Because of this, the most effective uses of the FADD marker are still undiscovered.

The new pre-associated FADD binding protein MYLK2 re-locates with the protein into the DISC. They share the same kinase domain and possess an inhibitory and a regulatory sequence. an inhibitory sequence. It has been established that a decrease in FADD recruitment could result in the development of cancer. However, this study is not conclusive and there are other potential uses for FADD-targeted mAbs.

High-affinity primary antibodies

Boster Bio manufactures IHC-optimized polyclonal antibodies as and picogram-sensitivity ELISA kit kits for biomarkers in cancer, biology and neurosciences, inflammation and physiology. Boster Bio's range of antibodies includes more than 12,000 high affinity primary antibodies. They are validated for WB and FC assays, as well IHC assays. The Boster Bio antibodies are tested against known quantities of recombinant proteins as well as untransfected cell lines to ensure high affinity.

In contrast to other companies, Boster Bio high-affinity primary antibodies are made of purified, quality-controlled, and tested antibodies. Boster antibodies have been utilized in research studies for more than 25 years and have been widely cited during this time. Boster also manufactures its ELISA kits and antibodies in-house and strictly adheres to quality control and efficiency standards. Boster antibodies can be purchased in liquid and solid-form, which permits researchers to test their choice on a variety of samples.

Researchers can quickly determine the proteins they require by using this high-affinity primary antibody. Boster Bio's high affinity antibodies are created by using the most modern methods that include site-directed mutation. These methods ensure high-specificity and low background. Boster Bio also provides customized services, and BeNeLux delivery. So, if you're looking for high-affinity primary antibodies Boster Bio is a leading source.

The major benefit of using this antibody is that it recognizes sporozoites when there are epitopes found on Pf sporozoites. Furthermore, since they do not have an epitope on their surfaces, mAb 5D5 cannot recognize the majority of live Pf sporozoites, leading to a high background signal. In addition the mAb 5D5 is unable to block the growth of mosquitoes or the invasion of hepatocytes.