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Product Info Summary
| SKU: | P00237 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-FADD (S194) Antibody
SKU/Catalog Number
P00237
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-FADD (S194) Antibody catalog # P00237. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-FADD (S194) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00237)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FADD around the phosphorylation site of Ser194. AA range:159-208
Reactive Species
P00237 is reactive to FADD in Human, Mouse
Observed Molecular Weight
52 kDa
Calculated molecular weight
23.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00237 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FADD (Phospho-Ser194) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of MOUSE-KIDNEY MOUSE-BRAIN MOUSE-SPLEEN MOUSE-LUNG using p-FADD (S194) antibody. Antibody was diluted at 1:1000
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Western blot analysis of lysates from HeLa cells treated with Paclitaxel 1uM 60', using FADD (Phospho-Ser194) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho-FADD (S194) Antibody (P00237)
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Customer Q&As
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7 Customer Q&As for Anti-Phospho-FADD (S194) Antibody
Question
I am interested in to test anti-Phospho-FADD (S194) antibody P00237 on human blood for research purposes, then I may be interested in using anti-Phospho-FADD (S194) antibody P00237 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The products we sell, including anti-Phospho-FADD (S194) antibody P00237, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-24
Question
Does anti-Phospho-FADD (S194) antibody P00237 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
According to the expression profile of blood, FADD is highly expressed in blood. So, it is likely that anti-Phospho-FADD (S194) antibody P00237 will work for WB with blood.
Boster Scientific Support
Answered: 2019-12-16
Question
Here is the WB image, lot number and protocol we used for blood using anti-Phospho-FADD (S194) antibody P00237. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-25
Question
I see that the anti-Phospho-FADD (S194) antibody P00237 works with WB, what is the protocol used to produce the result images on the product page?
S. Krishna
Verified customer
Asked: 2019-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-01-22
Question
I was wanting to use your anti-Phospho-FADD (S194) antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
It shows on the product datasheet, P00237 anti-Phospho-FADD (S194) antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-03
Question
My question regarding product P00237, anti-Phospho-FADD (S194) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00237 anti-Phospho-FADD (S194) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-26
Question
Would P00237 anti-Phospho-FADD (S194) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Moore
Verified customer
Asked: 2014-03-25
Answer
You can see on the product datasheet, P00237 anti-Phospho-FADD (S194) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-25
